Nguyen, Uyen Tran Tu Youn, Esther Le, Tram Anh Ngoc Ha, Ngoc Minh Tran, Son Hung Lee, Sohyun Cha, Jin Wook Park, Jin-Soo Kwon, Hak Cheol Kang, Kyungsu 2024-06-28T06:30:16Z 2024-06-28T06:30:16Z 2024-06-28 2024-08 0891-5849 https://pubs.kist.re.kr/handle/201004/150124 Photodynamic therapy is a noninvasive treatment in which specific photosensitizers and light are used to produce high amounts of reactive oxygen species (ROS), which can be employed for targeted tissue destruction in cancer treatment or antimicrobial therapy. However, it remains unknown whether lower amounts of ROS produced by mild photodynamic therapy increase lifespan and stress resistance at the organism level . Here, we introduce a novel photodynamic treatment (PDTr) that uses 20 mu M hypericin, a photosensitizer that originates from Hypericum perforatum , and orange light (590 nm, 5.4 W/m 2 , 1 min) to induce intracellular ROS formation (ROS), thereby resulting in lifespan extension and improved stress resistance in C. elegans . The PDTr-induced increase in longevity was abrogated by N -acetyl cysteine, suggesting the hormetic response was driven by prooxidative mechanisms. PDTr activated the translocation of SKN-1/NRF-2 and DAF-16/FOXO, leading to elevated expression of downstream oxidative stress -responsive genes, including ctl-1 , gst-4, and sod -3. In summary, our findings suggest a novel PDTr method that extends the lifespan of C. elegans under both normal and oxidative stress conditions through the activation of SKN-1 and DAF-16 via the involvement of many antioxidant genes. English Elsevier BV Photodynamic treatment increases the lifespan and oxidative stress resistance of Caenorhabditis elegans Article 10.1016/j.freeradbiomed.2024.05.023 1 Free Radical Biology and Medicine, v.221, pp.98 - 110 Free Radical Biology and Medicine 221 98 110 Y scie scopus 001244889800001 2-s2.0-85193469981 Biochemistry & Molecular Biology Endocrinology & Metabolism Biochemistry & Molecular Biology Endocrinology & Metabolism Article C-ELEGANS MITOHORMESIS RESTRICTION LONGEVITY THERAPY HEALTH AMPK Hypericin Photodynamic treatment Lifespan Hormesis Caenorhabditis elegans