Cho, Younghak Lee, Hana Jeong, Wonji Jung, Kwang Bo Lee, Sun Young Park, Seonghyeon Yeun, Jemin Kwon, Ohman Son, Jin Gyeong Lee, Tae Geol Son, Mi-Young Im, Sung Gap 2024-07-26T04:30:33Z 2024-07-26T04:30:33Z 2024-07-25 2024-09 0935-9648 https://pubs.kist.re.kr/handle/201004/150292 Human pluripotent stem cells (hPSCs), encompassing human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), hold immense potential in regenerative medicine, offering new opportunities for personalized cell therapies. However, their clinical translation is hindered by the inevitable reliance on xenogeneic components in culture environments. This study addresses this challenge by engineering a fully synthetic, xeno-free culture substrate, whose surface composition is tailored systematically for xeno-free culture of hPSCs. A functional polymer surface, pGC2 (poly(glycidyl methacrylate-grafting-guanidine-co-carboxylic acrylate)), offers excellent cell-adhesive properties as well as non-cytotoxicity, enabling robust hESCs and hiPSCs growth while presenting cost-competitiveness and scalability over Matrigel. This investigation includes comprehensive evaluations of pGC2 across diverse experimental conditions, demonstrating its wide adaptability with various pluripotent stem cell lines, culture media, and substrates. Crucially, pGC2 supports long-term hESCs and hiPSCs expansion, up to ten passages without compromising their stemness and pluripotency. Notably, this study is the first to confirm an identical proteomic profile after ten passages of xeno-free cultivation of hiPSCs on a polymeric substrate compared to Matrigel. The innovative substrate bridges the gap between laboratory research and clinical translation, offering a new promising avenue for advancing stem cell-based therapies. This study introduces a fully synthetic, xeno-free culture substrate, poly(glycidyl methacrylate-grafting-guanidine-co-carboxylicacrylate) (pGC2), engineered for human pluripotent stem cells cultivation. pGC2 supports robust growth of human pluripotent stem cells, maintaining stemness and pluripotency for up to ten passages, while offering cost-competitiveness and scalability over Matrigel. Comprehensive evaluations confirm its wide adaptability and potential for clinical translation in stem cell-based therapies. image English WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Long-Term Culture of Human Pluripotent Stem Cells in Xeno-Free Condition Using Functional Polymer Films Article 10.1002/adma.202403952 1 Advanced Materials, v.36, no.36 Advanced Materials 36 36 Y scie scopus 001268776900001 2-s2.0-85198614735 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article SELF-RENEWAL SURFACE DIFFERENTIATION ADHESION SYSTEM FEEDER GROWTH MATRIGEL COATINGS EXPANSION long-term culture pluripotency stemness xeno-free culture cell therapeutics human pluripotent stem cells initiated chemical vapor deposition