Kim, Seungil Nowicki, Kamil W. Kohyama, Keishi Mittal, Aditya Ye, Sangho Wang, Kai Fujii, Taro Rajesh, Shivbaskar Cao, Catherine Mantena, Rohit Barbuto, Marianna Jung, Youngmee Gross, Bradley A. Friedlander, Robert M. Wagner, William R. 2024-08-08T02:00:25Z 2024-08-08T02:00:25Z 2024-08-08 2024-08 1525-7797 https://pubs.kist.re.kr/handle/201004/150383 Cerebral aneurysms are a source of neurological morbidity and mortality, most often as a result of rupture. The most common approach for treating aneurysms involves endovascular embolization using nonbiodegradable medical devices, such as platinum coils. However, the need for retreatment due to the recanalization of coil-treated aneurysms highlights the importance of exploring alternative solutions. In this study, we propose an injectable extracellular matrix-derived embolic formed in situ by Michael addition of gelatin-thiol (Gel-SH) and hyaluronic acid vinyl sulfone (HA-VS) that may be delivered with a therapeutic agent (here, RADA-SP) to fill and remodel aneurysmal tissue without leaving behind permanent foreign bodies. The injectable embolic material demonstrated rapid gelation under physiological conditions, forming a highly porous structure and allowing for cellular infiltration. The injectable embolic exhibited thrombogenic behavior in vitro that was comparable to that of alginate injectables. Furthermore, in vivo studies in a murine carotid aneurysm model demonstrated the successful embolization of a saccular aneurysm and extensive cellular infiltration both with and without RADA-SP at 3 weeks, with some evidence of increased vascular or fibrosis markers with RADA-SP incorporation. The results indicate that the developed embolic has inherent potential for acutely filling cerebrovascular aneurysms and encouraging the cellular infiltration that would be necessary for stable, chronic remodeling. English American Chemical Society Development of an Injectable, ECM-Derivative Embolic for the Treatment of Cerebral Saccular Aneurysms Article 10.1021/acs.biomac.4c00321 1 Biomacromolecules, v.25, no.8, pp.4879 - 4890 Biomacromolecules 25 8 4879 4890 Y scie scopus 001279977600001 2-s2.0-85198601629 Biochemistry & Molecular Biology Chemistry, Organic Polymer Science Biochemistry & Molecular Biology Chemistry Polymer Science Article NEUROPEPTIDE SUBSTANCE-P SMOOTH-MUSCLE-CELLS ENDOVASCULAR TREATMENT TNF-ALPHA HYDROGELS EMBOLIZATION ANGIOGENESIS COILS