Ban, Seunghyun Suh, Hye-young Lee, Su Hyeon Kim, Si-Hong Oh, Sewon Jung, Je Hyeong 2024-08-23T08:30:23Z 2024-08-23T08:30:23Z 2024-08-22 2024-08 https://pubs.kist.re.kr/handle/201004/150479 Bud sports in fruit crops often result in new cultivars with unique traits, such as distinct fruit size and color, compared to their parent plants. This study investigates the phenotypic differences and gene expression patterns in Tonewase and Ohtanenashi persimmon bud sports compared to those in their parent, Hiratanenashi, based on RNA-seq data. Tonewase is characterized by early maturation, whereas Ohtanenashi is noted for its larger fruit size. Despite the importance of these traits in determining fruit quality, their molecular bases in persimmons have been understudied. We compared transcriptome-level differences during fruit development between the bud sport samples and their original cultivar. Comprehensive transcriptome analyses identified 15,814 differentially expressed genes and 26 modules via weighted gene co-expression network analysis. Certain modules exhibited unique expression patterns specific to the different cultivars during fruit development, likely contributing to the phenotypic differences observed. Specifically, M11, M16, M22, and M23 were uniquely expressed in Tonewase, whereas M13 and M24 showed distinct patterns in Ohtanenashi. By focusing on genes with distinct expression profiles, we aimed to uncover the genetic basis of cultivar-specific traits. Our findings suggest that changes in the expression of genes associated with ethylene and cell wall pathways may drive Tonewase's earlier maturation, whereas genes related to the cell cycle within the M24 module appear crucial for Ohtanenashi's larger fruit size. Additionally, ethylene and transcription factor genes within this module may contribute to the increased fruit size observed. This study elucidates the differences in transcriptomic changes during fruit development between the two bud sport samples and their original cultivar, enhancing our understanding of the genetic determinants influencing fruit size and maturation. English Frontiers Media S.A. Comparative transcriptome analysis of persimmon somatic mutants (Diospyros kaki) identifies regulatory networks for fruit maturation and size Article 10.3389/fpls.2024.1448851 1 Frontiers in Plant Science, v.15 Frontiers in Plant Science 15 Y scie scopus 001291960300001 Plant Sciences Plant Sciences Article MALUS X DOMESTICA MOLECULAR ANALYSIS CELL-DIVISION MUTATION GENE APPLE EXPRESSION ETHYLENE ROOT MAINTENANCE bud sports persimmon comparative transcriptome analysis fruit maturation fruit size