Raja, Iruthayapandi Selestin Kim, Chuntae Kang, Moon Sung Joung, Yoon Ki Lee, Jong Hun Han, Dong-Wook 2024-09-19T01:30:12Z 2024-09-19T01:30:12Z 2024-09-19 2024-09 2731-9229 https://pubs.kist.re.kr/handle/201004/150615 Functional nanocomposite-based printable inks impart strength, mechanical stability, and bioactivity to the printed matrix due to the presence of nanomaterials or nanostructures. Carbonaceous nanomaterials are known to improve the electrical conductivity, osteoconductivity, mechanical, and thermal properties of printed materials. In the current work, we have incorporated carbon nanofiber nanoparticles (CNF NPs) into methacrylated gelatin (GelMA) to investigate whether the resulting nanocomposite printable ink constructs (GelMA-CNF NPs) promote cell proliferation. Two kinds of printable constructs, cell-laden bioink and biomaterial ink, were prepared by incorporating various concentrations of CNF NPs (50, 100, and 150 mu g/mL). The CNF NPs improved the mechanical strength and dielectric properties of the printed constructs. The in vitro cell line studies using normal human dermal fibroblasts (nHDF) demonstrated that CNF NPs are involved in cell-material interaction without affecting cellular morphology. Though the presence of NPs did not affect cellular viability on the initial days of treatment, it caused cytotoxicity to the cells on days 4 and 7 of the treatment. A significant level of cytotoxicity was observed in the highly CNF-concentrated bioink scaffolds (100 and 150 mu g/mL). The unfavorable outcomes of the current work necessitate further study of employing functionalized CNF NPs to achieve enhanced cell proliferation in GelMA-CNF NPs-based bioprinted constructs and advance the application of skin tissue regeneration. English SPRINGER Studies on cytocompatibility of human dermal fibroblasts on carbon nanofiber nanoparticle-containing bioprinted constructs Article 10.1186/s11671-024-04110-9 1 Discover Nano, v.19, no.1 Discover Nano 19 1 Y scie scopus 001310863400001 Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Science & Technology - Other Topics Materials Science Physics Article HYDROGELS SCAFFOLDS GELATIN Carbon nanofiber nanoparticles Normal human dermal fibroblasts Skin tissue regeneration Nanocomposite bioinks