Cho, Yong Ho Kim, Seokho Won, Tae Kyung Cho, Sunki Ahn, Dong June 2024-10-10T08:00:20Z 2024-10-10T08:00:20Z 2024-10-10 2024-10 2590-0064 https://pubs.kist.re.kr/handle/201004/150781 This study introduces the time-gated analysis of room-temperature phosphorescence (RTP) for the in-situ analysis of the visible and spectral information of photons. Time-gated analysis is performed using a microscopic system consisting of a spectrometer, which is advantageous for in-situ analysis since it facilitates the realtime measurement of luminescence signal changes. An RTP material hybridized with a DNA aptamer that targets a specific protein enhances the intensity and lifetime of phosphorescence after selective recognition with the target protein. In addition, time-gated analysis allows for the millisecond-scale imaging of phosphorescence signals, excluding autofluorescence, and improves the signal-to-background ratio (SBR) through the accumulation of signals. While collecting the time-gated images and spectra of RTP and autofluorescent materials simultaneously, we develop a method for obtaining phosphorescence signals by means of selective exclusion of autofluorescence signals in simulated or real cell conditions. It is confirmed that the accumulated time-gated analysis can provide ample information about luminescence signals for bioimaging and biosensing applications. English Elsevier Accumulated in-situ spectral information analysis of room-temperature phosphorescence with time-gated bioimaging Article 10.1016/j.mtbio.2024.101238 1 Materials Today Bio, v.28 Materials Today Bio 28 Y scie scopus 001321615800001 2-s2.0-85203839514 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article VIVO FLUORESCENCE MICROSCOPY AUTOFLUORESCENCE PERSISTENT AFTERGLOW CRYSTALS CELLS Room-temperature phosphorescence (RTP) Time-gated microscopy In-situ spectroscopy Autofluorescence Signal accumulation