Joty, Kamruzzaman Ghimire, Madhav L. Kahn, Jason S. Lee, Sangyoup Alexandrakis, George Kim, Min Jun 2024-10-31T09:00:05Z 2024-10-31T09:00:05Z 2024-10-31 2024-11 0003-2700 https://pubs.kist.re.kr/handle/201004/150912 The rapidly advancing field of nanotechnology is driving the development of precise sensing methods at the nanoscale, with solid-state nanopores emerging as promising tools for biomolecular sensing. This study investigates the increased sensitivity of solid-state nanopores achieved by integrating DNA origami structures, leading to the improved analysis of protein translocations. Using holo human serum transferrin (holo-hSTf) as a model protein, we compared hybrid nanopores incorporating DNA origami with open solid-state nanopores. Results show a significant enhancement in holo-hSTf detection sensitivity with DNA origami integration, suggesting a unique role of DNA interactions beyond confinement. This approach holds potential for ultrasensitive protein detection in biosensing applications, offering advancements in biomedical research and diagnostic tool development for diseases with low-abundance protein biomarkers. Further exploration of origami designs and nanopore configurations promises even greater sensitivity and versatility in the detection of a wider range of proteins, paving the way for advanced biosensing technologies. English American Chemical Society DNA Origami Incorporated into Solid-State Nanopores Enables Enhanced Sensitivity for Precise Analysis of Protein Translocations Article 10.1021/acs.analchem.4c02016 1 Analytical Chemistry, v.96, no.44, pp.17496 - 17505 Analytical Chemistry 96 44 17496 17505 N scie scopus 001340234800001 Chemistry, Analytical Chemistry Article