SEO, YOON DAM Kang, In Seon Lee, Hyeon-Jeong Hwang, Ji In SON, Jung hyun Min-Kyu Oh Min, Ho phil 2024-11-04T12:00:24Z 2024-11-04T12:00:24Z 2024-10-28 2024-06-03 https://pubs.kist.re.kr/handle/201004/150946 Regular exercise induces multi-tissue adaptations impacting cardiovascular, muscle, bone, and joint health, influenced by genetic and environmental factors. However, research on the proteomic and metabolomic aspects of elite athletes' long-term exercise remains limited. To address this, we utilized LC-MS-based proteomics and metabolomics to investigate protein and metabolite expression across various sports disciplines and exercise types. For proteomic analysis, we employed the Data-Independent Acquisition (DIA) method, constructing an in-house spectral library from serum samples using Data-Dependent Acquisition (DDA) and Gas-Phase Fractionation (GPF)-DIA. Additionally, we optimized an analysis method for efficiently analyzing large-scale samples of athletes. By utilizing DIA analysis, we quantified approximately 400 proteins on average. Furthermore, we developed an 18-minute method capable of analyzing 80 samples per day, streamlining pre-treatment from 24 hours to 6 hours. For metabolomic analysis, we applied a large-scale targeted approach, screening over 300 substances in a 20-minute analysis, allowing faster processing of large samples compared to conventional methods. Utilizing these techniques, we conducted large-scale proteomic and metabolomic profiling of elite athletes. Our comprehensive analysis provides insights into the molecular mechanisms underlying long-term exercise and underscores the importance of integrated proteomic and metabolomic approaches in sports science. English American Society for Mass Spectrometry (ASMS) The impact of exercise on the serum proteomes and metabolomes of elite athletes participating in various sports disciplines Conference 1 American Society for Mass Spectrometry (ASMS) American Society for Mass Spectrometry (ASMS) US Anaheim Convention Center, CA 2024-06-02 American Society for Mass Spectrometry (ASMS)