Hwang, Ji In Lee, Hyeon-Jeong SEO, YOON DAM Eugene C. Yi Hwa Jeong Lee Min, Ho phil 2024-11-04T12:30:21Z 2024-11-04T12:30:21Z 2024-10-31 2024-06-02 https://pubs.kist.re.kr/handle/201004/150953 Current treatments for muscle degeneration focus on diet, but TGF-β pathway manipulation (myostatin, activin A, GDF-11) shows promise. WADA bans these inhibitors for athletes. This study developed a detection method for target monoclonal antibodies (Landogrozumab, Domagrozumab, Bimagrumab, targeting TGF-β and Dulaglutide as a Internal standard. Using dried blood spots (DBS) and protein G magnetic beads for purification, followed by LC-MS/MS analysis. FAIMS was applicated to increase sensitivity of target unique peptides. Assays showed strong linearity (r = 0.99) and no interference issues. The matrix effect was near 100%, confirming DBS suitability for analysis. Though these antibodies lack clinical approval, this detection method is vital for doping control. English American Society for Mass Spectrometry (ASMS) Simultaneous detection method of muscle developing monoclonal antibodies with using LC-MS/MS from Dried Blood Spots Conference 1 American Society for Mass Spectrometry (ASMS) American Society for Mass Spectrometry (ASMS) US Anaheim Convention Center, CA 2024-06-02 American Society for Mass Spectrometry (ASMS)