Keum, Changjoon Yeom, Haejin Noh, Tae Il Yi, Seung Yong Jin, Soyeong Kim, Chaekyu Shim, Ji Sung Yoon, Sung Goo Kim, Hojun Lee, Kwan Hyi Kang, Seok Ho Jeong, Youngdo 2024-12-13T01:30:37Z 2024-12-13T01:30:37Z 2024-12-12 2025-07 2157-846X https://pubs.kist.re.kr/handle/201004/151365 Diagnostic kits for the optical detection of bladder cancer in urine can facilitate effective screening and surveillance. However, the heterogeneity of urine samples, owing to patients with bladder cancer often presenting with haematuria, interfere with the transduction of the optical signal. Here we describe the development and point-of-care performance of a device for the detection of bladder cancer that obviates the need for sample processing. The device leverages the enzymatic release of organogel particles carrying solvatochromic fluorophores in the presence of urinary hyaluronidases-a bladder cancer biomarker. Owing to buoyancy, the particles transfer from the urine sample into the organic phase, where the change in fluorescence can be measured via a smartphone without interference from blood proteins. In a double-blind study with 80 unprocessed urine samples from patients with bladder cancer (including samples with haematuria) or other genitourinary diseases and with 25 samples from healthy participants, our system distinguished the cancerous samples, including those with early-stage bladder cancer, with accuracies of about 90%. Obviating the need for sample pretreatment may facilitate the at-home detection of bladder cancer. A point-of-care diagnostic device for the detection of early-stage bladder in urine obviates the need of sample pretreatment and enhances diagnostic accuracy. English NATURE PUBLISHING GROUP Diagnosis of early-stage bladder cancer via unprocessed urine samples at the point of care Article 10.1038/s41551-024-01298-0 1 Nature Biomedical Engineering, v.9, pp.1026 - 1038 Nature Biomedical Engineering 9 1026 1038 N scie scopus 001366131200001 2-s2.0-85210505122 Engineering, Biomedical Engineering Article HYALURONIC-ACID ACCURACY MARKERS BIOMARKERS HEMATURIA