Cha, Jaehyun Choi, Youngjun Sung, Changmin Kim, Minyoung Park, Won-Kun Min, Kyoungseon 2025-01-07T06:00:09Z 2025-01-07T06:00:09Z 2024-12-30 2025-02 0960-8524 https://pubs.kist.re.kr/handle/201004/151494 To overcome the climate crisis, various greenhouse gas (GHG) mitigation strategies have been developed, and every effort has been made to achieve carbon neutrality. Given that petroleum-based industries and the transportation sector emit enormous amounts of GHGs, the product spectra of biorefineries should be expanded beyond drop-in biofuels to include more value-added products. This study aimed to construct a CO2 mitigation system. When CO2 was used as the sole feedstock, value-added phycocyanin and enantioselective gamma-valerolactone (GVL) were produced as follows (i) Galdieria sulphuraria was phototrophically cultured to produce phycocyanin (ii) the residual G. sulphuraria after phycocyanin extraction was used as the feedstock for producing levulinic acid and formic acid and (iii) the engineered hydroxybutyrate dehydrogenase-based chemo-enzymatic reaction converted levulinic acid to (R)-GVL with perfect enantiomeric excess > 99.999 %. The results not only contribute to broadening the product spectrum of CO2 biorefineries but also provide strategies to achieve carbon neutrality. English Elsevier BV Bio-mitigation of CO2: The co-production of phycocyanin and optically pure (R)-γ-valerolactone with perfect enantiomeric excess Article 10.1016/j.biortech.2024.131895 1 Bioresource Technology, v.418 Bioresource Technology 418 N scie scopus 001374723600001 2-s2.0-85210545231 Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels Agriculture Biotechnology & Applied Microbiology Energy & Fuels Article LEVULINIC ACID REDUCTION Phycocyanin CO2 biorefinery Galdieria sulphuraria Engineered hydroxybutyrate dehydrogenase Optically pure (R)-gamma-valerolactone