Muthusamy, Selvaraj Rajalakshmi, Kanagaraj Kannanalanisamy, P Ahn, Dae-Hwan Zhu, Dongwei Nam, Yun-Sik Choi, Ki Young Meng, Suci Luo, Zhibin Song, Jong-Won Xu, Yuanguo 2025-01-07T07:30:04Z 2025-01-07T07:30:04Z 2025-01-03 2025-03 0925-4005 https://pubs.kist.re.kr/handle/201004/151501 Cancer diagnosis accurately greatly depends on the ability to differentiate between cancerous and normal cells and tissues during the early stages of the disease, which are frequently accompanied by abnormalities in the intracellular hydrogen sulfide (H2S) and microenvironment (viscosity). Herein, we designed a multi-responsive probe (TL) for the simultaneous detection of H2S and viscosity. Firstly, TL reacted with D (2,4-dinitrophenyl) and observed the intramolecular charge transfer sensing mechanism as well as TL-D, which was developed together with fluorescence quenching at 558？nm. Since D is an H2S-recognizing unit, the probe-attached D (TL-D) could react with H2S spontaneously, reproducing TL in reversible processes besides achieving quantitative emission from 558 to 496？nm. Following that, the suggested mechanism was confirmed by examining H2S and viscosity using the newly synthesized probe TL-D. Remarkably, probe TL-D can be employed for simultaneous imaging of H2S, and viscosity in dual channels has also been accomplished in non-alcoholic fatty liver (NAFL) disease liver tissues in addition to cancer cells and NAFL mouse models. As anticipated, for visual clinical diagnosis, tracking many biomarkers simultaneously across multiple channels appeared to be more reliable than recognizing a single biomarker. English Elsevier BV A dual-channel fluorescent probe for simultaneous imaging of H2S and viscosity in non-alcoholic fatty liver and tumor tissue models Article 10.1016/j.snb.2024.137216 1 Sensors and Actuators, B: Chemical, v.427 Sensors and Actuators, B: Chemical 427 N scie scopus 001399342300001 Chemistry, Analytical Electrochemistry Instruments & Instrumentation Chemistry Electrochemistry Instruments & Instrumentation Article LARGE STOKES SHIFT HYDROGEN-SULFIDE FOOD SPOILAGE Tetraphenylethene H 2 S/viscosity Non-alcoholic fatty liver Tumor tissue Cancer diagnosis