Hong, Soyeon Lee, Hee Ju Jung, Da Seul Erdenebileg Saruul Hwang, Ho Seong Kwon, Hak Cheol Kwon, Jaeyoung Yoo, GyHye 2025-01-13T09:30:08Z 2025-01-13T09:30:08Z 2025-01-09 2025-01 https://pubs.kist.re.kr/handle/201004/151546 Background: Osteoporosis is characterized by the microstructural depletion of bone tissue and decreased bone density, leading to an increased risk of fractures. Cotoneaster wilsonii Nakai, an endemic species of the Korean Peninsula, grows wild in Ulleungdo. In this study, we aimed to investigate the effects of C. wilsonii and its components on osteoporosis. Methods and Results: The alkaline phosphatase (ALP) activity of C. wilsonii extracts and fractions was evaluated in MC3T3-E1 pre-osteoblasts, and the n-hexane fraction (CWH) showed the best properties for ALP activity. The effects of the CWH on bone formation were assessed in MC3T3-E1 cells and ovariectomized mice. Biochemical assays and histological analyses focused on the signaling activation of osteoblast differentiation and osteogenic markers, such as ALP, collagen, and osterix. The CWH significantly activated TGF-β and Wnt signaling, enhancing osteoblast differentiation and bone matrix formation. Notably, CWH treatment improved micro-CT indices, such as femoral bone density, and restored serum osteocalcin levels compared to OVX controls. Conclusions: These results highlight the potential of the C. wilsonii Nakai n-hexane fraction as a promising therapeutic agent for managing osteoporosis. English Multidisciplinary Digital Publishing Institute (MDPI) Exploring the Anti-Osteoporotic Effects of n-Hexane Fraction from Cotoneaster wilsonii Nakai: Activation of Runx2 and Osteoblast Differentiation In Vivo Article 10.3390/ph18010045 1 Pharmaceuticals, v.18, no.1 Pharmaceuticals 18 1 Y scie scopus 001403825400001 Chemistry, Medicinal Pharmacology & Pharmacy Pharmacology & Pharmacy Article BONE-FORMATION POSTMENOPAUSAL OSTEOPOROSIS BETA-SITOSTEROL MECHANISM CELLS <italic>Cotoneaster wilsonii</italic> Nakai bone formation osteoblast differentiation osteoporosis phytomedicine ovariectomized mice