Lee, So Jeong Kim, Jiwon Ahn, Jung Ho Gong, Gyeongtaek Um, Youngsoon Lee, Sun-Mo Kim, Kyoung Heon Ko, Ja Kyong 2025-01-17T05:00:05Z 2025-01-17T05:00:05Z 2025-01-15 2025-02 0960-8524 https://pubs.kist.re.kr/handle/201004/151563 Lignocellulosic biomass is a promising renewable feedstock for biodegradable plastics like polyhydroxyalkanoates (PHAs). Cupriavidus necator, a versatile microbial host that synthesizes poly(3-hydroxybutyrate) (PHB), the most abundant type of PHA, has been studied to expand its carbon source utilization. Since C. necator NCIMB11599 cannot metabolize xylose, we developed xylose-utilizing strains by introducing synthetic xylose metabolic pathways, including the xylose isomerase, Weimberg, and Dahms pathways. Through rational and evolutionary engineering, the RXI22 and RXW62 strains were able to efficiently utilize xylose as the sole carbon source, producing 64.2 wt% (wt%) and 61.4 wt% PHB, respectively. Among the engineered strains, the xylose isomerase-based RXI22 strain demonstrated the most efficient co-fermentation performance, with a PHB content of 75.7 wt% and a yield of 0.32 (g PHB/g glucose and xylose) from mixed sugars. The strains developed in this study represent an enhanced PHA producer, offering a sustainable route for converting lignocellulosic biomass into bioplastics. English Elsevier BV Engineering xylose utilization in Cupriavidus necator for enhanced poly(3-hydroxybutyrate) production from mixed sugars Article 10.1016/j.biortech.2024.131996 1 Bioresource Technology, v.418 Bioresource Technology 418 N scie scopus 001393953100001 Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels Agriculture Biotechnology & Applied Microbiology Energy & Fuels Article NITRIC-OXIDE OPTIMIZATION METABOLISM H16 ESCHERICHIA-COLI DAHMS PATHWAY Biodegradable polymer Metabolic engineering Lignocellulosic sugar Xylose isomerase pathway Weimberg pathway