이지연 김진우 장판식 김진철 2025-01-20T02:30:05Z 2025-01-20T02:30:05Z 2025-01-17 2024-12 0367-6293 https://pubs.kist.re.kr/handle/201004/151613 This study aimed to evaluate the impact of calcium hydroxide supplement (CHS) on wound healing using human keratinocytes and elucidate its underlying cellular mechanisms. Although CHS did not directly promote human keratinocyte (HaCaT) migration, it exhibited a wound healing effect on HaCaT cells in CHS-treated human dermal fibroblast (HDF)-conditioned media (CM). Compared with cells treated with HDF-CM alone, cell migration was significantly increased (37.6%) upon treatment with 1.6 μg/mL of CHS-treated HDF-CM. Treatment with CHS-treated HDF-CM significantly activated GS3k-β/β-catenin signaling pathways, which are key mediators of cell proliferation. These results indicate that CHS possesses wound healing potential and provide a molecular basis for the development of functional natural products for wound healing. Korean 한국식품과학회 수산화 칼슘 보충제의 피부세포 증식 향상을 통한 상처 치유 효과 Calcium hydroxide supplementation promotes wound healing of HaCaT keratinocytes by enhancing cell proliferation Article 10.9721/KJFST.2024.56.6.741 1 Korean Journal of Food Science and Technology, v.56, no.6, pp.741 - 746 Korean Journal of Food Science and Technology 56 6 741 746 N scopus kci ART003146708 calcium hydroxide cell proliferation keratinocyte fibroblast wound healing