Jeon, Hyungju Kim, Jiwon Kim, Jayoung Choi, Yoon Kyoung Ho, Chun Lum Andy Pifferi, Fabien Huber, Daniel Feng, Linqing Kim, Jinhyun 2025-02-04T08:00:05Z 2025-02-04T08:00:05Z 2025-02-04 2024-12 0027-8424 https://pubs.kist.re.kr/handle/201004/151707 The gray mouse lemur (Microcebus murinus), one of the smallest living primates, emerges as a promising model organism for neuroscience research. This is due to its genetic similarity to humans, its evolutionary position between rodents and humans, and its primate-like features encapsulated within a rodent-sized brain. Despite its potential, the absence of a comprehensive reference brain atlas impedes the progress of research endeavors in this species, particularly at the microscopic level. Existing references have largely been confined to the macroscopic scale, lacking detailed anatomical information. Here, we present eLemur, a unique resource, comprising a repository of high-resolution brain-wide images immunostained with multiple cell type and structural markers, elucidating the cyto- and chemoarchitecture of the mouse lemur brain. Additionally, it encompasses a segmented two-dimensional reference and 3D anatomical brain atlas delineated into cortical, subcortical, and other vital regions. Furthermore, eLemur includes a comprehensive 3D cell atlas, providing densities and spatial distributions of non-neuronal and neuronal cells across the mouse lemur brain. Accessible via a web-based viewer (https://eeum-brain.com/#/lemurdatasets), the eLemur resource streamlines data sharing and integration, fostering the exploration of different hypotheses and experimental designs using the mouse lemur as a model organism. Moreover, in conjunction with the growing 3D datasets for rodents, nonhuman primates, and humans, our eLemur 3D digital framework enhances the potential for comparative analysis and translation research, facilitating the integration of extensive rodent study data into human studies. English National Academy of Sciences eLemur: A cellular-resolution 3D atlas of the mouse lemur brain Article 10.1073/pnas.2413687121 1 Proceedings of the National Academy of Sciences of the United States of America, v.121, no.50 Proceedings of the National Academy of Sciences of the United States of America 121 50 N scie scopus 001378563200001 Multidisciplinary Sciences Science & Technology - Other Topics VISUAL-CORTEX PRIMATE MODEL NEUROSCIENCE ONTOLOGY BIOLOGY VGLUT2 ADULT Gray mouse lemur ( Microcebus murinus) 3D brain atlas cellular resolution website resources