Ji, Moongi Park, Jiyeon Choi, Byeongchan Lee, Hyeon-Seong Seo, Chan Park, Meejung Lee, Sooyeun Paik, Man-Jeong 2025-03-22T11:30:10Z 2025-03-22T11:30:10Z 2025-03-19 2026-01 0003-2719 https://pubs.kist.re.kr/handle/201004/151975 γ-Hydroxybutyric acid (GHB) is a central nervous system depressant with high addiction risk, causing oxidative stress and neurotoxicity. Urinary nucleoside metabolomics studies in single- and multiple- administration rats have not been performed. Most nucleoside levels were higher in the single-administered group than the multiple-administered group. Multivariate analysis identified cytidine, pseudouridine, uridine, N4-acetylcysteine, adenosine, and 5’-deoxy-5’-methylthioadenosine as significant for group discrimination in principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). These metabolites performed well in receiver operating characteristic (ROC) analysis, suggesting they could be potential biomarkers for GHB exposure and addiction. English Marcel Dekker Inc. Urinary Metabolomics Study of Nucleosides in Rats Following Intraperitoneal Injection of γ-Hydroxybutyric Acid Article 10.1080/00032719.2025.2463410 1 Analytical Letters, v.59, no.1, pp.15 - 29 Analytical Letters 59 1 15 29 N scie scopus 001420357100001 2-s2.0-85218209395 Chemistry, Analytical Chemistry Article GHB BRAIN gamma-hydroxybutyric acid liquid chromatography-tandem mass spectrometry metabolomics nucleoside profiling analysis rat urine