Kwon, Eun Jung Sung, Hyeon Mi Lee, Hansong Ahn, Soyul Kim, Yejin Lee, Chae Rin Kim, Kihun Myung, Kyungjae Kim, Won Kyu Kim, Dokyoung Jeong, Sanghwa Oh, Chang-Kyu Kim, Yun Hak 2025-03-22T12:00:28Z 2025-03-22T12:00:28Z 2025-03-19 2025-01 https://pubs.kist.re.kr/handle/201004/151982 Polystyrene nanoparticles pose significant toxicological risks to aquatic ecosystems, yet their impact on zebrafish ( Danio rerio) embryonic development, particularly erythropoiesis, remains underexplored. This study used single-cell RNA sequencing to comprehensively evaluate the effects of polystyrene nanoparticle exposure on erythropoiesis in zebrafish embryos. In vivo validation experiments corroborated the transcriptomic findings, revealing that polystyrene nanoparticle exposure disrupted This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http:// creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. erythrocyte differentiation, as evidenced by the decrease in mature erythrocytes and concomitant increase in immature erythrocytes. Additionally, impaired heme synthesis further contributed to the diminished erythrocyte population. These findings underscore the toxic effects of polystyrene nanoparticles on hematopoietic processes, highlighting their potential to compromise organismal health in aquatic environments. English Science Press Deciphering the toxic effects of polystyrene nanoparticles on erythropoiesis at single-cell resolution Article 10.24272/j.issn.2095-8137.2024.277 1 Zoological Research, v.46, no.1, pp.165 - 176 Zoological Research 46 1 165 176 Y scie scopus 001408425500013 2-s2.0-85216607616 Zoology Zoology Article MICROPLASTICS NANOPLASTICS Polystyrene nanoparticles Zebrafish embryos Single-cell RNA sequencing Erythropoiesis