Kulikouskaya, Viktoryia Hileuskaya, Kseniya Chekanouskaya, Lizaveta Jezhora, Maryia Kraskouski, Aliaksandr Pinchuk, Sergei Vasilevich, Irina Ladutska, Alena Melnikova, Galina Vasilkevich, Vadim Bogdanov, Ruslan Saichuk, Anastasiia Ihnatsyeu-Kachan, Aliaksei Kim, Sehoon Dudchik, Natallia Volotovski, Igor Agabekov, Vladimir 2025-03-22T12:30:09Z 2025-03-22T12:30:09Z 2025-03-19 2025-04 0254-0584 https://pubs.kist.re.kr/handle/201004/151985 Multifunctional ultrathin coatings are of great interest in biomedical-related fields in order to impart the desired properties, such as biocompatibility and antimicrobial or anti-inflammatory activities, to the surfaces of bio-implantable materials. Herein, a deep insight into the features of the design of chitosan-Ag/pectin-Ag and chitosan-Ag/pectin multilayer films using layer-by-layer (LbL) assembly was considered in detail. It has been determined that film growth regime (linear or parabolic) and elastic properties can be controlled by changing the type of polyanionic component in the multilayer system and the number of bilayers, respectively. The obtained films were characterized using QCM-D, AFM, and XPS. Contact angle measurements and tribological testing were also carried out. LbL films possessed a uniform defect-free surface (roughness <11 nm), excellent hydrophilic properties (water contact angle 45.9 divided by 61.7 degrees), and good wear resistance. A comprehensive study of the biological properties of LbL films was carried out. It has been demonstrated that multilayer systems exhibited excellent antibacterial activity against B. subtilis (up to 4.1 log reduction) and E. coli (up to 3.9 log reduction) and a considerable radical-scavenging effect (ABTS(center dot+) inhibition reached 27.1 %). LbL films did not induce inflammation and can demonstrate anti-inflammatory action (reduction of induced IL-1 beta level up to 2.6 times) depending on the structure of chitosan-Ag used as the polycation component. LbL films demonstrated high resistance to nonspecific protein adsorption and good biocompatibility with mesenchymal stem cells (the number of viable cells similar to 90 %). An in vivo toxicity study indicated that the obtained multilayer systems do not show acute oral and intraperitoneal toxicity; the irritation action and allergenic activity were also not observed. Our results indicate the prospects of applying LbL films for implant modification based on the complex of their physicochemical and biological properties. English Elsevier BV Engineering the ultrathin coatings with multifunctional bioactivity based on chitosan-Ag and pectin-Ag nanocomposites for modification of bio-implantable materials Article 10.1016/j.matchemphys.2025.130443 1 Materials Chemistry and Physics, v.334 Materials Chemistry and Physics 334 N scie scopus 001414017900001 2-s2.0-85215780376 Materials Science, Multidisciplinary Materials Science Article QUARTZ-CRYSTAL MICROBALANCE POLYELECTROLYTE MULTILAYERS SILVER NANOPARTICLES CITRUS PECTIN LINEAR GROWTH POLYMER-FILMS QCM-D ANTIBACTERIAL ANTIOXIDANT ADSORPTION Layer-by-Layer assembly Polysaccharide-Ag nanoparticles Antibacterial Antioxidant Biocompatibility