Kim, Hyewon Kim, Taeyeon Rahaman, Khandoker Asiqur Kwon, Jieun Lee, Ji-Young Cho, Seohyeon Chung, Seok Han, Hyung-Seop Kim, Yu-Chan 2025-04-09T09:00:24Z 2025-04-09T09:00:24Z 2025-04-09 2025-06 1047-4838 https://pubs.kist.re.kr/handle/201004/152239 Regeneration of the central and peripheral nervous system is challenging because of the inherent low regenerative capacity. Transplantation of neural stem cells (NSCs) is a promising strategy to increase regenerative capacity, but limited viability, slow proliferation, and differentiation create significant hurdles. Combining neurostimulator bio-functional ions such as magnesium (Mg2+) and zinc (Zn2+) to mitigate this limitation could be a breakthrough for future nerve regeneration treatments. This is the first study to investigate the effects of combined Mg2+ and Zn2+ ion therapy on the proliferation and differentiation of NSCs. The results showed an optimal concentration of Mg2+ and Zn2+ ions individually improved NSC proliferation, with a synergistic effect observed when ions were combined. The synergistic effect also significantly enhanced neurite outgrowth, promoted advanced neuronal network formation, and increased Nestin, beta III-tubulin, and MAP2 gene expression. These findings highlight the potential of combination therapy of Mg2+ and Zn2+ ions, which can be used as complementary modulators with NSCs-based treatments, providing promising ion-based therapeutic strategies in neural repair and regenerative medicine. English J O M Institute Synergistic Effects of Magnesium and Zinc Ions on Neural Stem Cell Proliferation and Differentiation Article 10.1007/s11837-025-07330-1 1 JOM, v.77, no.6, pp.4402 - 4413 JOM 77 6 4402 4413 N scie scopus 001454873300001 Materials Science, Multidisciplinary Metallurgy & Metallurgical Engineering Mineralogy Mining & Mineral Processing Materials Science Metallurgy & Metallurgical Engineering Mineralogy Mining & Mineral Processing Article ELEVATION IN-VITRO ADULT