Cha, Yujin Song, Yeonju Jung, Cheulhee Ko, Yeon jin Lee, Min Hee 2025-04-25T07:30:29Z 2025-04-25T07:30:29Z 2025-04-25 2025-08 0925-4005 https://pubs.kist.re.kr/handle/201004/152330 Cancer biomarkers, including hydrogen sulfide (H2S) and human NAD(P)H quinone oxidoreductase-1 (hNQO1), are critical indicators of tumor presence and progression, offering valuable insights for diagnosis, prognosis, and treatment response. H2S, a gasotransmitter that is overexpressed in various cancers, promotes tumor progression and metastasis, while hNQO1, an enzyme involved in cellular redox balance, is upregulated in multiple cancers and associated with poor patient outcomes. Here, we report the development of a novel dual-responsive fluorescent probe, azide-coumarin-trimethyl lock quinone (ACT), designed to selectively detect both H2S and hNQO1 activity in live cells. ACT remains minimally fluorescent under physiological conditions but exhibits a strong fluorescence upon co-recognition of H2S and hNQO1. This dual-targeting probe enables the distinction between cancerous and normal cells, underscoring its potential as a sensitive and selective diagnostic tool for cancers characterized by the co-overexpression of these biomarkers. English Elsevier BV A dual-responsive fluorescent probe for cancer diagnostics via simultaneous detection of H2S and hNQO1 Article 10.1016/j.snb.2025.137682 1 Sensors and Actuators, B: Chemical, v.436 Sensors and Actuators, B: Chemical 436 N scie scopus 001460724800001 2-s2.0-105001339972 Chemistry, Analytical Electrochemistry Instruments & Instrumentation Chemistry Electrochemistry Instruments & Instrumentation Article NQO1 HYDROGEN-SULFIDE hNQO1 Cancer diagnostics Colorectal cancer Dual-responsive fluorescent probe H2S