Kim, Eun Jae Kwon, Eunjung Oh, Seo Jung Choi, Mi Ran Lee, Sang-Rae Jung, Byung Hwa Lee, Wonwoong Hong, Jongki 2025-04-25T08:00:49Z 2025-04-25T08:00:49Z 2025-04-25 2025-05 0021-9673 https://pubs.kist.re.kr/handle/201004/152342 The use of electronic cigarettes (e-cigarettes) has gained popularity worldwide for reducing the unpleasant odors and flavors of smoking marijuana. However, due to the high vaporization temperature of the heating coil in ecigarettes, cannabinoids could be converted into secondary cannabinoid products, potentially causing unintended psychological and harmful effects. A lab-built impinger and aerosol collection device was prepared to study the thermal transformation of cannabinoids during e-cigarette vaping, optimizing collection conditions according to variations in coil wattage, cartridge oil, and collection solvents. Thermal conversion of individual cannabidiolic acid (CBDA), cannabidiol (CBD), and Delta 9-tetrahydrocannabinol (Delta 9-THC) in e-cartridge liquid was performed with increasing coil power from 45 W to 105 W. Collected aerosol solution was derivatized with trimethylsilyl reagents and analyzed by gas chromatography-mass spectrometry (GC-MS) scan mode. Thermal vaping profiles of individual authentic cannabinoids were studied according to the variation of coil power of the e-cigarette. During the CBDA vaping process of the e-cigarette, most of the acidic CBDA was converted to neutral CBD through thermal decarboxylation and further degraded to produce several thermal products. Several interesting psychoactive Delta 8-iso and Delta 9-THC isomers, and cannabichromene (CBC) and CBD quinone (CBDQ) were observed from the vaping process of CBDA and CBD. In the case of Delta 9-THC vaping, psychoactive hexahydrocannabinol (HHC) derivatives and cannabinol (CBN), were produced via thermal reduction and oxidation. These thermal products were identified by comparing retention times and mass spectra of authentic standards and interpreting their mass spectra. The amounts of most thermal products were increased with increasing coil power from 45 W to 105 W. In contrast, potentially harmful CBDQ was found to be highest amount at 45 W and decreased with increasing coil power. From the profile data and identification results, thermal transformation pathways of cannabinoids during the vaping process are proposed. This study will provide important information on the formation mechanism of thermal conversion products and basic guidance for risk assessment of Cannabis oil vaping by e-cigarette. English Elsevier BV Thermal transformation of CBD, CBDA, and Δ9-THC during e-cigarette vaping: Identification of conversion products by GC-MS Article 10.1016/j.chroma.2025.465909 1 Journal of Chromatography A, v.1749 Journal of Chromatography A 1749 N scie scopus 001458252200001 2-s2.0-105001284513 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article CANNABINOIDS CANNABIDIOL Cannabinoid Lab-built vaping device Thermal transformation Psychoactive products GC-MS