Jeong, Tae Yeong Yoon, Da Eun Kim, Sol Pin Yang, Jiyun Lim, Soo-Yeon Ok, Sungjin Ju, Sungjin Park, Jeongeun Lee, Su Bin Park, Soo-Ji Kim, Sanghun Lee, Hyunji Lee, Daekee Kang, Soo Kyung Lee, Seung Eun Kim, Hyeon Soo Seong, Je Kyung Kim, Kyoungmi 2025-05-09T05:30:10Z 2025-05-09T05:30:10Z 2025-05-07 2025-04 https://pubs.kist.re.kr/handle/201004/152377 Genetically engineered mouse models (GEMMs) are crucial for investigating disease mechanisms, developing therapeutic strategies, and advancing fundamental biological research. While CRISPR gene editing has greatly facilitated the creation of these models, existing techniques still present technical challenges and efficiency limitations. Here, we establish a CRISPR-VLP-induced targeted mutagenesis (CRISPR-VIM) strategy, enabling precise genome editing by co-culturing zygotes with virus-like particle (VLP)-delivered gene editing ribonucleoproteins (RNPs) without requiring physical manipulation or causing cellular damage. We generate Plin1- and Tyr-knockout mice through VLP-based SpCas9 or adenine base editor (ABE)/sgRNA RNPs and characterize their phenotype and germline transmission. Additionally, we demonstrate cytosine base editor (CBE)/sgRNA-based C-to-T substitution or SpCas9/sgRNA-based knock-in using VLPs. This method further simplifies and accelerates GEMM generation without specialized techniques or equipment. Consequently, the CRISPR-VIM method can facilitate mouse modeling and be applied in various research fields. English Nature Publishing Group An innovative approach using CRISPR-ribonucleoprotein packaged in virus-like particles to generate genetically engineered mouse models Article 10.1038/s41467-025-58364-7 1 Nature Communications, v.16, no.1 Nature Communications 16 1 Y scie scopus 001465538200013 2-s2.0-105002964915 Multidisciplinary Sciences Science & Technology - Other Topics Article TARGET BASE EFFICIENT ELECTROPORATION DELIVERY MICE MUTAGENESIS PERILIPIN CELLS CAS9 PROTEIN GENOMIC DNA