Jeong, Jae-Seung Ju, Hyunsu Cho, Chi-Hyun 2025-05-11T05:00:42Z 2025-05-11T05:00:42Z 2025-05-07 2025-04 1449-1907 https://pubs.kist.re.kr/handle/201004/152398 This study measures expression of nuclear factor kappa B (NF-kappa B)1 and related cytokine genes in bone marrow mononuclear cells in patients with hematological malignancies, analyzing the relationship between them with an integrated framework of statistical analyses, machine learning (ML), and explainable artificial intelligence (XAI). While traditional dimensionality reduction techniques-such as principal component analysis, linear discriminant analysis, and t-distributed stochastic neighbor embedding-showed limited differentiation embedding, ML classifiers (k-Nearest Neighbors, Na & iuml;ve Bayes Classifier, Random Forest, and XGBoost) successfully identified critical patterns. Notably, normalized caspase-1 counts consistently emerged as the most influential feature associated with NF-kappa B1 activity across disease groups, as highlighted by SHapley Additive exPlanations analyses. Systematic evaluation of ML performance on small datasets revealed that a minimum sample size of 15-24 is necessary for reliable classification outcomes, particularly in cohorts of acute myeloid leukemia and myelodysplastic syndrome. These findings underscore the pivotal role of caspase-1 to the NF-kappa B1 gene expression in hematologic malignancy diseases. Furthermore, this study demonstrates the feasibility of leveraging ML and XAI to derive meaningful insights from limited data, offering a robust strategy for biomarker discovery and precision medicine in rare hematological malignancies. English Ivyspring International Publisher Analysis of the Relationship Between NF-κB1 and Cytokine Gene Expression in Hematological Malignancy: Leveraging Explained Artificial Intelligence and Machine Learning for Small Dataset Insights Article 10.7150/ijms.109493 1 International Journal of Medical Sciences, v.22, no.9, pp.2208 - 2226 International Journal of Medical Sciences 22 9 2208 2226 Y scie scopus 001472594000005 Medicine, General & Internal General & Internal Medicine Article NF-KAPPA-B INFLAMMASOMES MECHANISM TRANSCRIPTION FACTORS INFLAMMATION CLASSIFICATION INTERLEUKIN-1 SUBUNIT DISEASE CANCER NF-kappa B Hematological Malignancy Machine Learning Classifiers Explainable Artificial Intelligence Small Data Adaptation