Kim, Yu-Jin Kim, Hyeok Lee, Dong-Hyun Kim, Yeong Hwan Park, Jae-Hyun Sim, Woo-Sup Kim, Jin-Ju Ban, Kiwon Um, Soong Ho Park, Hyun Ji Davis, Michael E. Park, Hun-Jun Bhang, Suk Ho 2025-05-11T05:30:49Z 2025-05-11T05:30:49Z 2025-05-07 2025-08 https://pubs.kist.re.kr/handle/201004/152403 Despite the promising potential of stromal cell therapy in treating myocardial infarction (MI), its effectiveness is limited by poor cell retention and engraftment in ischemic environments. This study introduces a novel strategy that combines the preconditioning of human adipose-derived stromal cells (hADSCs) using OLED-based photobiomodulation (OPBM) and culturing these cells into 3D spheroids. The preconditioned 3D spheroids (APCS group) exhibit significantly enhanced angiogenic, arterialized, and tissue remodeling capabilities compared with those of traditional 2D cultures and non-preconditioned spheroids. In vivo transplantation of these spheroids into the border zone of infarcted area significantly improve cardiac function and reduce adverse remodeling by enhancing anti-fibrosis and angiogenesis including arterialization. The combined strategy with OPBM preconditioning and 3D spheroid culture system can enhance therapeutic potential of hADSCs with multiple paracrine effects for cardiac repair. This novel approach provides next generation of cell therapeutics to overcome the limitation of adult stromal cell therapy in patients with post-MI heart failure. English Wiley-VCH Verlag Reinforcing Stromal Cell Spheroid Through Red-Light Preconditioning for Advanced Vascularization Article 10.1002/advs.202500788 1 Advanced Science, v.12, no.29 Advanced Science 12 29 Y scie scopus 001474947500001 Chemistry, Multidisciplinary Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science Article MESENCHYMAL STEM-CELLS ENDOTHELIAL GROWTH-FACTOR INDUCIBLE FACTOR-I REGENERATIVE CELLS ARTERIOGENESIS ISCHEMIA ANGIOGENESIS ENGRAFTMENT PROMOTE REPAIR angiogenesis arteriogenesis myocardial infarction OPBM-preconditioning stromal cell spheroid