Lee, Yoon-Jin Seo, Chul Won Chae, Shinwon Lee, Chang Yeol Kim, Sang Soo Shin, Yoon-Hee Park, Hyun-Mee Gho, Yong Song Ryu, Seongho Lee, Sang-Han Choi, Dongsic 2025-05-11T07:00:13Z 2025-05-11T07:00:13Z 2025-05-07 2025-04 1535-9476 https://pubs.kist.re.kr/handle/201004/152421 Most cancer cells adopt a less efficient metabolic process of aerobic glycolysis with high level of glucose uptake followed by lactic acid production, known as the Warburg effect. This phenotypic transition enables cancer cells to achieve increased cellular survival and proliferation in a harsh low-oxygen tumor microenvironment. Also, the resulting acidic microenvironment causes inactivation of the immune system such as T-cell impairment that favors escape by immune surveillance. While lots of studies have revealed that tumor-derived EVs can deliver parental materials to adjacent cells and contribute to oncogenic reprogramming, their functionality in energy metabolism is not well addressed. In this study, we established prostate cancer cells PC-3AcT resistant to cellular death in an acidic culture medium driven by lactic acid. Quantitative proteomics between EVs derived from PC-3 and PC-3AcT cells identified 935 confident EV proteins. According to cellular adaptation to lactic acidosis, we revealed 159 regulated EV proteins related to energy metabolism, cellular shape, and extra-cellular matrix. These EVs contained a high abundance of glycolytic enzymes. In particular, PC-3AcT EVs were enriched with apolipoproteins including apolipoprotein B-100 (APOB). APOB on PC-3AcT EVs could facilitate their endocytic uptake depending on low density lipoprotein receptor of recipient PC-3 cells, encouraging increases of cellular proliferation and survival in acidic culture media via increased activity and expression of hexokinases and phosphofructokinase. The activation of recipient PC-3 cells can increase glucose consumption and ATP generation, representing an acquired metabolic reprogramming into the Warburg phenotype. Our study first revealed that EVs derived from prostate cancer cells could contribute to energy metabolic reprogramming and that the acquired metabolic phenotypic transition of recipient cells could favor cellular survival in tumor microenvironment. English American Society for Biochemistry and Molecular Biology Inc. Metabolic Reprogramming Into a Glycolysis Phenotype Induced by Extracellular Vesicles Derived From Prostate Cancer Cells Article 10.1016/j.mcpro.2025.100944 1 Molecular & Cellular Proteomics, v.24, no.4 Molecular & Cellular Proteomics 24 4 Y scie scopus 001468266500001 2-s2.0-105002781865 Biochemical Research Methods Biochemistry & Molecular Biology Article HEXOKINASE-II SURFACE HISAT