Park, Tae-Jin Choi, Byeong Min Hong, Hyehyun Park, Jin-Soo Kim, Seung-Young 2025-05-22T06:31:24Z 2025-05-22T06:31:24Z 2025-05-21 2025-04 1017-7825 https://pubs.kist.re.kr/handle/201004/152498 We investigated the potential of Ajuga decumbens Thunb. as an anti-inflammatory agent by utilizing plant resources to develop materials through the application of tissue culture and light-emitting diode (LED) cultivation technologies. To compare the changes between A. decumbens callus extract (ADCB) cultivated under blue monochromatic LED light and ADC (the negative control) cultivated under dark conditions, their morphological characteristics were tested and LC/MS analyses were conducted. ADCB exhibited a greenish hue compared with ADC and contained increased levels of specific compounds. The anti-inflammatory activities of the two samples were evaluated using LPSstimulated macrophages. None of the samples exhibited cytotoxicity at any tested concentration. However, ADCB demonstrated a greater ability to reduce nitric oxide and key pro-inflammatory cytokines including interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha compared to the control. Furthermore, ADCB effectively suppressed the expression of inducible nitric oxide synthase and cyclooxygenase-2. It inhibited the phosphorylation of mitogen-activated protein kinase family proteins, including extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38, in a concentration-dependent manner. Tissue culture and LED cultivation technologies are significant methods for addressing plant supply challenges and enhancing the content of bioactive compounds, thereby increasing the applicability of plant materials. Moreover, ADCB produced using these technologies exhibited anti-inflammatory activity without causing irritation to human skin at active concentrations, suggesting its potential as a novel anti-inflammatory material. English 한국미생물·생명공학회 Anti-Inflammatory Effects of Ajuga decumbens Extract under Blue Light Stimulation Article 10.4014/jmb.2502.02002 1 Journal of Microbiology and Biotechnology, v.35 Journal of Microbiology and Biotechnology 35 Y scie scopus kci 001481436000017 2-s2.0-105004333616 Biotechnology & Applied Microbiology Microbiology Biotechnology & Applied Microbiology Microbiology Article CHRONIC INFLAMMATION SIGNALING PATHWAY CELL-DEATH EXPRESSION CALLUS PGE(2) COX-2 Ajuga decumbens LED anti-inflammatory plant callus MAPK