Yu, Subin Kang, Haeun Jee, Seohyeon Moon, Wooyeon Jang, Dohyub Huang, Wen-Tse Kim, Dongjun Chung, Kyungwha Won, Dong-Il Park, Jungwon Liu, Ru-Shi Choi, Kyungmin Kim, Sehoon Lee, Luke P. Kim, Dong Ha 2025-06-05T02:30:18Z 2025-06-05T02:30:18Z 2025-06-04 2025-07 2192-2640 https://pubs.kist.re.kr/handle/201004/152567 Metal-organic frameworks (MOFs) are widely used as substrates for creating single-atom catalysts due to their abundance of ligands, facilitating enzyme-like activity for biomedical applications. However, the high-temperature calcination process for single-atom catalysts limits economical, efficient, and large-scale synthesis. Here, a simple room-temperature synthesis of MOF-based single-atom and metal cluster catalysts is presented for tumor therapy. Fe/MOF is obtained through a coordination reaction at room temperature, while Au/MOF is synthesized from Au3+/MOF by introducing a reducing agent. Au/MOF effectively generates hydrogen peroxide (H2O2) from glucose, outperforming Au3+/MOF, and Fe/MOF subsequently produced hydroxyl radicals (center dot OH) by decomposing the generated H2O2via accelerated peroxidase-like activity in an acidic environment. In vitro and in vivo studies confirm a significantly enhanced cancer eradication ability compared to the PBS-treated group by combining cascade enzymatic activity, destruction of oxidative homeostasis, and excessive mitochondrial-mediated lipid peroxidation. The novel synthesis process of MOF-based metal single-atom catalysts establishes a new paradigm for fabricating effective enzyme-like nanomaterials for multimodal tumor therapy. English Wiley-Blackwell MOF-Based Single-Atom and Metal Cluster Catalysts by Room-Temperature Synthesis for Tumor Therapy Article 10.1002/adhm.202501058 1 Advanced Healthcare Materials, v.14, no.18 Advanced Healthcare Materials 14 18 N scie scopus 001495399800001 Engineering, Biomedical Nanoscience & Nanotechnology Materials Science, Biomaterials Engineering Science & Technology - Other Topics Materials Science Article ORGANIC FRAMEWORKS FERROPTOSIS NANOZYMES ASSAY glucose oxidase mimic metal-organic framework peroxidase mimic single atom catalyst tumor therapy