Le, Huong-Giang Lee, Yongjae Lee, Sun-Mi 2025-06-19T02:00:16Z 2025-06-19T02:00:16Z 2025-06-13 2025-03 1225-8873 https://pubs.kist.re.kr/handle/201004/152633 The increasing environmental concerns regarding conventional plastics have led to a growing demand for sustainable alternatives, such as biodegradable plastics. Yeast cell factories, specifically Saccharomyces cerevisiae and Yarrowia lipolytica, have emerged as promising platforms for bioplastic production due to their scalability, robustness, and ease of manipulation. This review highlights synthetic biology approaches aimed at developing yeast cell factories to produce key biodegradable plastics, including polylactic acid (PLA), polyhydroxyalkanoates (PHAs), and poly (butylene adipate-co-terephthalate) (PBAT). We explore recent advancements in engineered yeast strains that utilize various synthetic biology strategies, such as the incorporation of new genetic elements at the gene, pathway, and cellular system levels. The combined efforts of metabolic engineering, protein engineering, and adaptive evolution have enhanced strain efficiency and maximized product yields. Additionally, this review addresses the importance of integrating computational tools and machine learning into the Design-Build-Test-Learn cycle for strain development. This integration aims to facilitate strain development while minimizing effort and maximizing performance. However, challenges remain in improving strain robustness and scaling up industrial production processes. By combining advanced synthetic biology techniques with computational approaches, yeast cell factories hold significant potential for the sustainable and scalable production of bioplastics, thus contributing to a greener bioeconomy. English 한국미생물학회 Synthetic biology strategies for sustainable bioplastic production by yeasts Article 10.71150/jm.2501022 1 The Journal of Microbiology, v.63, no.3 The Journal of Microbiology 63 3 N scie scopus kci ART003187553 001501992100002 2-s2.0-105003193573 Microbiology Microbiology Review L-LACTIC ACID SACCHAROMYCES-CEREVISIAE ESCHERICHIA-COLI ADIPIC ACID BIOTECHNOLOGICAL PRODUCTION EFFICIENT PRODUCTION POLYLACTIC ACID PHA SYNTHASE BIOSYNTHESIS POLYHYDROXYBUTYRATE yeast synthetic biology bioplastics PLA PBAT PHA