Park, Sung Hun Lee, Jaewon Kim, ChaeEon Kang, Byungsoo Gopinath, Ashwin Lee, Seungwoo 2025-06-24T02:30:26Z 2025-06-24T02:30:26Z 2025-06-23 2025-07 https://pubs.kist.re.kr/handle/201004/152680 DNA origami (DO) enables the precise spatial arrangement of guest components through sequence-specific hybridization. Its deterministic placement onto lithographically defined chemical patches-termed DO placement (DOP)-allows their integration into device architectures. However, conventional DOP has primarily been limited to oxide-based substrates, restricting its compatibility with broader device platforms. Herein, we demonstrate the selective binding of DO onto patterned gold (Au) substrates as a foundation for DOP on metallic substrates. Leveraging the versatility of microcontact printing of self-assembled monolayers (SAMs), we patterned Au substrates with regions of distinct surface potential energies. Initially, we employed monofunctionalized SAMs to explore the influence of surface chemistry and molecular density on DO binding. Monte Carlo simulations provided blueprints for the selective binding probability of DO as a function of the SAM composition and order-state. Guided by these insights, we then utilized bifunctionalized SAMs to achieve high-yield, site-selective DO binding on targeted regions of Au substrates. English AMER CHEMICAL SOC Selective Binding of DNA Origami on Self-Assembled Monolayer-Patterned Gold Substrate Article 10.1021/acsmaterialslett.5c00533 1 ACS Materials Letters, v.7, no.7, pp.2565 - 2573 ACS Materials Letters 7 7 2565 2573 N scie scopus 001505608500001 2-s2.0-105007868527 Materials Science, Multidisciplinary Materials Science Article CRYSTALS SUPERRESOLUTION MICROSCOPY FOLDING DNA FABRICATION ALKANETHIOLS NANOANTENNAS WETTABILITY ORIENTATION ADSORPTION PLACEMENT