Jo, Seung-Woo Velankanni, Priyanka Cam, Nhung Do Thi Nguyen, Chau Hoang Bao Jeon, Jin-Young Kim, Bo-Ra Lee, Dae-Hee Lee, Choong-Gu Park, Jin-Soo 2025-07-18T02:30:23Z 2025-07-18T02:30:23Z 2025-07-18 2025-06 1017-7825 https://pubs.kist.re.kr/handle/201004/152755 Chlorella, a genus of single-celled green algae, is widely industrially cultivated as a dietary supplement ingredient due to its high protein content and various nutrients. In this study, the immune-stimulating effect of solvent fractions prepared from Chlorella vulgaris (CV) and Chlorella protothecoides (CP) was evaluated through the expression of immune-related factors, such as TNF-alpha and IFN-gamma in T cells obtained from mice. Both Chlorella species showed immunostimulatory effects in hexane and butanol fractions, but CV increased TNF-alpha only, while CP elevated both TNF-alpha and IFN-gamma. In addition, the lipid profile of Chlorella was characterized using non-selective LC-MS/MS-based metabolomic analysis. According to the lipidomic results, the two species differed in lipid composition, with PC 32:1, PC 32:2, PC 34:5, DGDG 34:4, and DGDG 34:5 being enriched in the active fractions of CV, and PC 34:1, PC 34:2, PC O-15:2 and DGTS 34:4 being abundant in the fractions of CP. Furthermore, 5'-methylthioadenosine (MTA), which was detected in both Chlorella species, also exhibited immunostimulatory properties. English 한국미생물·생명공학회 Chemical Profiling and Immune-Stimulating Activity of Solvent Fractions Derived from Dietary Chlorella Article 10.4014/jmb.2503.03021 1 Journal of Microbiology and Biotechnology, v.35, pp.1 - 8 Journal of Microbiology and Biotechnology 35 1 8 N scie scopus kci ART003222009 001515313100013 Biotechnology & Applied Microbiology Microbiology Biotechnology & Applied Microbiology Microbiology Article LIPID PRODUCTION VULGARIS 5&apos -METHYLTHIOADENOSINE POLYSACCHARIDES MACROPHAGES CELLS Chlorella vulgaris Chlorella protothecoides immune-stimulating activity lipid 5’-methylthioadenosine (MTA)