Lee, Donggeun Jeon, Seung-Woo Yi, Chang-Hwan Kim, Yanghee Choi, Yeeun Lee, Sang-Hun Cha, Jinwoong Shim, Seung-Bo Suh, Junho Kim, Il-Young Kang, Dongyeon Daniel Jung, Hojoong Jeong, Cherlhyun Ahn, Jae-pyoung Park, Hee Chul Han, Sang-Wook Kim, Chulki 2025-07-18T06:30:28Z 2025-07-18T06:30:28Z 2025-07-18 2025-07 1530-6984 https://pubs.kist.re.kr/handle/201004/152777 The significance of mass spectrometry lies in its unparalleled ability to accurately identify and quantify molecules in complex samples, providing invaluable insights into molecular structures and interactions. Here, we leverage diamond nanostructures as highly sensitive mass sensors by utilizing a self-excitation mechanism under an electron beam in a conventional scanning electron microscope (SEM). The diamond molecular balance (DMB) exhibits a practical mass resolution of 4.07 MDa, based on its notable mechanical quality factor and frequency stability, along with a broad dynamic range from MDa to TDa. This positions the DMB at the forefront of nanoelectromechanical system (NEMS)-based mass spectrometry operating at room temperature. Notably, the DMB demonstrated its ability to measure the mass of a single bacteriophage T4 by precisely locating the analyte on the device. These findings demonstrate the capability and potential of the DMB as a revolutionary tool for mass spectrometry at room temperature. English American Chemical Society Diamond Molecular Balance: Ultra-Wide Range Nanomechanical Mass Spectrometry from MDa to TDa Article 10.1021/acs.nanolett.5c02032 1 Nano Letters, v.25, no.26, pp.10497 - 10503 Nano Letters 25 26 10497 10503 Y scie scopus 001518543100001 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article Diamond Nanoelectromechanical Systems MassSpectrometry Bacteriophage T4 Multiplexing4