Bae, Won-Young Nguyen, Uyen Tran Tu Le, Tram Anh Ngoc Tran, Son Hung Lee, Sohyun Hong, Sung-Chul Cha, Kwang Hyun Choi, Il-Dong Kang, Kyungsu 2025-07-18T07:00:22Z 2025-07-18T07:00:22Z 2025-07-18 2025-07 2042-650X https://pubs.kist.re.kr/handle/201004/152789 Caenorhabditis elegans is widely used as a model for investigating longevity owing to its short life cycle and the presence of human orthologs. This study aimed to ultimately prolong the lifespan of C. elegans by evaluating the gastrointestinal tract conditions and intestinal permeability of C. elegans fed Lacticaseibacillus casei HY2782 alone or fermented fecal products. The anti-inflammatory effects of L. casei HY2782 were determined based on interleukin 8 (IL-8) levels and intestinal permeability in human intestinal epithelial cells. In the C. elegans model, intestinal permeability was assessed in the N2 wild-type as well as skn-1, pmk-1, daf-16, and aak-2 mutant worms. During simulated colonic fecal fermentation, changes in short chain fatty acid and microbial composition were investigated. L. casei HY2782 reduced IL-8 production and intestinal permeability from 1646.8 to 1009.1 pg mL(-1) and 265.5 to 115.1%, respectively (p < 0.01). Additionally, L. casei HY2872 attenuated intestinal leakage in C. elegans and prolonged its lifespan via the DAF-16/FOXO and SKN-1/NRF2 pathways and gst-4 gene expression. Moreover, L. casei HY2782 inhibited intestinal leakage in aged worms. During fermentation, L. casei HY2782 produced butyrate under both normal and high-protein conditions. Additionally, L. casei HY2782 contributed to butyrate production by genera, such as Faecalibacterium and Lachnospira (p < 0.01), while inhibiting Fusobacterium (p < 0.05). L. casei HY2782 also prolonged lifespan of intestinally damaged worms (p < 0.001). Furthermore, C. elegans fed L. casei HY2782-fermented fecal products lived significantly longer than those fed the vehicle control (p < 0.001). Overall, L. casei HY2782 restored the intestinal tract by mitigating inflammation and microbial metabolic dysbiosis, ultimately extending the lifespan of C. elegans. English Royal Society of Chemistry Lacticaseibacillus casei HY2782 improves the intestinal barrier and tract environment and ultimately prolongs the lifespan of Caenorhabditis elegans Article 10.1039/d5fo01239b 1 Food & Function, v.16, no.14, pp.5871 - 5884 Food & Function 16 14 5871 5884 Y scie scopus 001519808700001 Biochemistry & Molecular Biology Food Science & Technology Biochemistry & Molecular Biology Food Science & Technology Article DIET