Yang, Da Som Zhou, Mingyu Li, Shupeng Cho, Soongwon Wu, Yixin Sekine, Yurina Kanatzidis, Evangelos E. Lv, Zengyao Yamagishi, Kento Kim, Joohee Park, Chanho Yoo, Seonggwang Hua, Hanbang Park, Jihun Kang, Jiheon Kim, Jong Uk Feng, Jingyuan Pniushkova, Ekaterina Kwon, Kyeongha Kim, Jae-Hwan Seo, Seong Gi Kwak, Jay Young Avila, Raudel Senzaki, Takahiro Aranyosi, Alexander J. Ghaffari, Roozbeh Huang, Yonggang Rogers, John 2025-07-31T02:00:24Z 2025-07-31T02:00:24Z 2025-07-28 2026-01 1616-301X https://pubs.kist.re.kr/handle/201004/152907 Emerging classes of wearable microfluidic sensors of eccrine sweat enable non-invasive and real-time monitoring of health status in applications that range from sports to worker safety, environmental exposures, and medical care. Opportunities for improving the performance of these systems include those in colorimetric detection of biochemical species with high accuracy across a wide dynamic range, in efficient collection of sweat and local measurements of sweat loss across a broad spectrum of sweat rates, and in an expanded set of biomarker targets. This paper introduces concepts in 3D microfluidic structures, surface chemistry, interface design, and colorimetric chemical reagents that address these opportunities. Demonstrations involve accurate quantification of sweat rate and total loss over a wide range, as well as precise colorimetric sensing of chloride, xanthine, and creatinine, as biomarkers associated with electrolyte balance, purine metabolism, and kidney function, respectively. Benchtop evaluations confirm high sensitivity and reproducibility, while on-body trials illustrate reliable biomarker tracking in response to dietary intake and physiological activity. These ideas and chemistries expand the possibilities in sweat-based diagnostic strategies, in strategies that have the potential to align with established, high-volume manufacturing practices for commercial production. English John Wiley & Sons Ltd. Soft, Skin-Interfaced 3D Microfluidic Systems for High Performance Assessment of Sweat Rate, Cumulative Loss and Biochemical Content Article 10.1002/adfm.202509169 1 Advanced Functional Materials, v.36, no.2 Advanced Functional Materials 36 2 N scie 001529927100001 2-s2.0-105010957649 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article CYSTIC-FIBROSIS CREATINE SUPPLEMENTATION BODY DIAGNOSIS EXERCISE ASSOCIATION PILOCARPINE SECRETION SKELETAL-MUSCLE METABOLISM STAGE RENAL-DISEASE sweat sensors wearable sensors microfluidics colorimetric sensing