Won, Jihoon Cho, Seunghye Kim, Kyoung-Ran Kim, Sehoon Ahn, Dae-Ro 2025-09-17T02:02:17Z 2025-09-17T02:02:17Z 2025-09-16 2025-10 0169-409X https://pubs.kist.re.kr/handle/201004/153168 DNA nanostructures have emerged as programmable and biocompatible platforms for drug delivery, offering precise control over size, shape, and surface properties. Recent advances have demonstrated their potential for organ-targeted delivery by utilizing ligand conjugation, structural engineering, and modulation of protein corona composition. Despite their promise, key challenges remain in predicting organ specificity and ensuring structural stability in vivo. This review provides a comprehensive overview of DNA nanostructures that have demonstrated organ-specific drug delivery, with emphasis on biodistribution profiles, in vivo targeting strategies, and the influence of physicochemical and biological barriers. We also highlight recent insights into corona-assisted targeting and administration route-dependent distribution, outlining strategies to enhance translational potential. Finally, we discuss critical challenges and future directions for clinical application of DNA nanostructures as targeted nanocarriers. English Elsevier BV Engineering in vivo behavior of DNA nanostructures toward organ-targeted drug delivery Article 10.1016/j.addr.2025.115682 1 Advanced Drug Delivery Reviews, v.225 Advanced Drug Delivery Reviews 225 Y scie scopus 001566532200001 2-s2.0-105014727693 Pharmacology & Pharmacy Pharmacology & Pharmacy Review FRAMEWORK NUCLEIC-ACIDS PROTEIN CORONA ORIGAMI NANOSTRUCTURES BIOLOGICAL IDENTITY NANOPARTICLES BRAIN OLIGONUCLEOTIDES DESIGN BIODISTRIBUTION NANOTECHNOLOGY DNA nanostructures Organ-targeted delivery Protein corona Biodistribution Systemic administration