Kim, Tae-Hun Jung, Jaeyong Sung, Jeong Soo Kwon, Soonil Bae, Hyung Eun Shim, Won-Bo Kang, Min-Jung Lee, Do Young Jose, Joachim Pyun, Jae-Chul 2025-09-17T02:02:25Z 2025-09-17T02:02:25Z 2025-09-16 2026-01 1976-0280 https://pubs.kist.re.kr/handle/201004/153169 A one-step immunoassay for influenza A detection was established based on screened Fv-antibodies and switching peptides. The switching peptides were synthesized to bind at the frame regions of Fv-antibodies and they dissociated upon binding of target analytes at Fv-antibodies. As the one-step assay could be performed without washing steps and additional reagents treatments, the assay result could be obtained just by mixing samples within 30 min. In this study, Fv-antibodies specific to the influenza virus were screened from an Fv-antibody library expressed on the outer membrane of E. coli BL21(DE3). From the Fv-antibody library, two Fv-antibodies against influenza A nucleoprotein were selected and produced as soluble proteins. The two produced Fv-antibodies specific to the Influenza nucleoprotein had binding constants of 211 and 497 nM. The diagnostic potential of the one-step immunoassay was demonstrated by detecting clinically relevant influenza A and B virus samples. The one-step immunoassay, enabled detection of both influenza A and B. English 한국바이오칩학회 One-Step Immunoassay of Influenza Virus Using Screened Fv-Antibodies and Switching Peptides Article 10.1007/s13206-025-00238-7 1 BioChip Journal, v.20, no.1, pp.87 - 100 BioChip Journal 20 1 87 100 N scie scopus kci ART003302842 001563953900001 Biochemical Research Methods Chemistry, Analytical Nanoscience & Nanotechnology Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Article INFECTION DIAGNOSIS IMMUNOSENSOR BIOSENSOR DOCKING ASSAY Influenza virus Nucleoprotein Fv-antibody Switching peptide One-step immunoassay