김형민 최원석 박혜원 윤인찬 윤태영 한성민 2025-09-29T02:30:24Z 2025-09-29T02:30:24Z 2025-09-22 2025-05-09 https://pubs.kist.re.kr/handle/201004/153253 Conventional methods for generating spheroids or organoids often rely on cell-type-dependent self-assembly and lack precise control over size and shape, which are critical for gradient, differentiation, and reproducibility. Recently, non-invasive methods using physical forces such as magnetic and optical fields have been explored to assemble single cells into 3D structures with tunable dimensions. Among them, ultrasound has emerged as a promising technique due to its label-free, compatibility with various cell types, and minimal biological damage. In this study, we propose a method to fabricate dimension-tunable 3D cellular blocks using phase-delayed acoustic vortices. Acoustic vortices generate a null-pressure region that traps cells and facilitates their aggregation into structured blocks. By adjusting acoustic parameters such as pressure, frequency, and sonication time, the size and shape of the blocks can be precisely controlled. The blocks remained structurally stable for 7 days, and viability assays confirmed that cell viability was well maintained without significant adverse effects. This technique offers a precise and flexible way to make 3D cell structures for research and biomedical applications. Korean 대한의용생체공학회 음향 소용돌이를 이용한 치수 조절이 가능한 세포 블록의 제작 Fabrication of Dimension-tunable Cellular Blocks via Acoustic Vortex Conference 2 2025년도 제 65회 대한의용생체공학회 춘계학술대회 2025년도 제 65회 대한의용생체공학회 춘계학술대회 KO 제주도 롯데 호텔(중문) 2025-05-08 2025년도 제 65회 대한의용생체공학회 춘계학술대회