Kim, Min Kyeong Yoon, Jungbin Kim, Dohui Kim, Jongmin Kim, Jae-Seok Go, Gyugang Song, Junhyeok Son, Seunghun Park, Kyungdeok Lee, Jooyoung Lee, Donghan Cho, Sumin Choi, Wonseok Min, Jaehong Cho, Young-Sam Lee, Seung-Jae Yi, Hee-Gyeong Choi, Andrew Jeon, Seunggyu Lee, Hyungseok Choi, Dongwhi Han, Sungmin Ko, Jihoon Cho, Dong-woo Kim, Dong Sung Jang, Jinah Kang, Hyun-Wook 2025-11-06T05:00:42Z 2025-11-06T05:00:42Z 2025-11-06 2025-09 2234-7593 https://pubs.kist.re.kr/handle/201004/153404 Advanced biomanufacturing technologies are rapidly transforming the development of microphysiological systems (MPS), which serve as sophisticated in vitro platforms to model human organ structure and function with high fidelity. This review highlights cutting-edge biofabrication strategies, including 3D bioprinting technologies (such as inkjet, extrusion-based, digital light processing, stereolithography, and laser direct writing), microfluidics, modular tissue engineering, and electrohydrodynamic manufacturing that enable precise fabrication of complex, multicellular, and physiologically relevant tissue models. The integration of microfluidic systems enhances MPS by supporting dynamic perfusion, mechanical stimulation, and real-time monitoring, while modular approaches such as cell spheroid, organoid, and cell sheet assembly facilitate scalable and reproducible tissue engineering. Electrohydrodynamic techniques like electrospinning and melt electrowriting are emphasized for their ability to fabricate nanostructured scaffolds that closely mimic native extracellular matrix properties. This review also examines the selection and application of biomaterials, ranging from natural and synthetic polymers to hybrid composites and stimuli responsive hydrogels, that underpin the structural and functional integrity of MPS. Finally, the broad applications of advanced biomanufactured MPS in drug screening, toxicology, disease modeling, and regenerative medicine are discussed, emphasizing their potential to reduce reliance on animal models and accelerate biomedical discoveries toward clinical translation. The convergence of real-time sensing, smart materials, and modular design principles is identified as a key driver for the next generation of physiologically relevant and patient specific in vitro models. English 한국정밀공학회 Advanced Biomanufacturing Technologies for Micro-physiological Systems Article 10.1007/s12541-025-01330-6 1 International Journal of Precision Engineering and Manufacturing, v.26, no.9, pp.2411 - 2462 International Journal of Precision Engineering and Manufacturing 26 9 2411 2462 Y scie scopus kci 001587277700001 2-s2.0-105017975806 Engineering, Manufacturing Engineering, Mechanical Engineering Review HYALURONIC-ACID HYDROGELS FULL-THICKNESS WOUNDS HUMAN SKIN EQUIVALENT IN-VITRO CELL SHEETS EXTRACELLULAR-MATRIX SAFETY ASSESSMENT TRACT EPITHELIUM ON-A-CHIP CULTURED EPITHELIAL AUTOGRAFTS