Lee, Jiyeon Kim, Jisun Bong, Ki Wan Song, Soo-Chang 2025-11-14T07:32:55Z 2025-11-14T07:32:55Z 2025-11-11 2025-11 2047-4830 https://pubs.kist.re.kr/handle/201004/153456 Ensuring a stable oxygen supply for transplanted cells remains a major challenge in the clinical translation of tissue engineering and regenerative medicine. Hypoxic environments caused by insufficient vascularization are a key factor leading to cell death and graft failure. To address this issue, we developed an injectable, oxygen-generating thermoresponsive hydrogel system based on poly(organophosphazene) (PPZ). By modulating the gelatin and calcium peroxide (CaO2) content, we fabricated calcium peroxide-loaded (CPO) microspheres with distinct oxygen release profiles and incorporated them into the PPZ hydrogel, forming a hydrogel based oxygen delivery platform, termed OxyCellgel. This platform, composed solely of PPZ and CPO microspheres, allows for precise control over oxygen release rates and amounts, enabling adaptation to both mild and severe hypoxic environments. The interaction between the microspheres and hydrogel matrix facilitated uniform and sustained oxygen release. Subsequently, human mesenchymal stem cells (hMSCs) were co-delivered with this OxyCellgel system to evaluate cell viability and function under hypoxic conditions. The system significantly enhanced the survival and proliferation of hMSCs and promoted angiogenesis through their paracrine effects under hypoxia. Notably, hMSCs co-encapsulated with OxyCellgel showed markedly improved viability under hypoxic conditions compared to controls. This study presents a hydrogel-based oxygen delivery platform with controllable release kinetics as a promising strategy to improve the efficacy of stem cell-based therapies under diverse hypoxic conditions. English Royal Society of Chemistry Modulating oxygen release via manipulated microspheres embedded in thermoresponsive hydrogels for enhanced stem cell survival under hypoxia Article 10.1039/d5bm00480b 1 Biomaterials Science, v.13, no.22, pp.6326 - 6349 Biomaterials Science 13 22 6326 6349 N scie scopus 001584903300001 2-s2.0-105018749958 Materials Science, Biomaterials Materials Science Article IN-VITRO GELATIN MICROSPHERES INDUCED APOPTOSIS DIFFERENTIATION ANGIOGENESIS SYSTEM VASCULARIZATION MICROPARTICLES BONE-MARROW STRATEGIES