Sung, Min Kyung Heo, Jin Young Jang, Seonhye Kim, Hansol Jeong, Youngdo Lee, Sang-Jae Kim, Seong-Bo Kim, Sung Tae 2025-11-26T10:31:07Z 2025-11-26T10:31:07Z 2025-11-26 2025-12 1226-086X https://pubs.kist.re.kr/handle/201004/153683 Nanostructured lipid carriers (NLCs) are the second generation of lipid nanoparticles for delivering pharmaceutical drugs. The current study aimed to improve the cellular uptake of NLCs by preparing surface functionalization. In this study, surface-functionalized NLCs were prepared by conjugating affibody molecules targeting HER2 or the cell-penetrating peptide TAT using the SpyTag/SpyCatcher system for enhanced cellular uptake. The cellular uptake and internalization mechanisms of functionalized NLCs were evaluated. NLCs were successfully prepared with nanosized lipid particles (<160 nm) and maintained storage stability, which was assessed by particle size, polydispersity index, and zeta potential values. Functionalized NLCs with HER2 affibody or TAT peptide were endocytosed when taken up by various cell lines, including SK-BR-3, MCF-7, MDA-MB-231, and HeLa, which were evaluated by flow cytometry and confocal microscopy analysis. These results demonstrated that surface functionalization significantly enhanced the cellular uptake and concomitant cytotoxicity of the NLCs. These findings suggest that the SpyTag/SpyCatcher-based strategy is effective for surface functionalization of lipid-based drug carriers. English 한국공업화학회 SpyTag/SpyCatcher-ligated nanostructured lipid carriers for active mammary cancer targeting Article 10.1016/j.jiec.2025.05.017 1 Journal of Industrial and Engineering Chemistry, v.152, pp.488 - 496 Journal of Industrial and Engineering Chemistry 152 488 496 N scie scopus kci 001598149300031 2-s2.0-105005189279 Chemistry, Multidisciplinary Engineering, Chemical Chemistry Engineering Article AFFIBODY MOLECULES CELLULAR UPTAKE DRUG-DELIVERY TAT PEPTIDE NANOPARTICLES PROTEIN NLC MECHANISMS SYSTEM SLN Nanostructured lipid carrier SpyTag/SpyCatcher Surface functionalization Doxorubicin Mammary cancer cell