Moussa, Hissein Hassan Sonibare, Mubo Adeola Park, Jin-Soo 2025-12-19T06:31:00Z 2025-12-19T06:31:00Z 2025-12-19 2025-11 1017-7825 https://pubs.kist.re.kr/handle/201004/153797 The rising need for new antibiotics and antioxidants highlights endophytic bacteria as promising sources of bioactive compounds. Medicinal plants such as Azadirachta indica harbour diverse endophytes, yet their potential in southwest Nigeria remains largely underexplored. This study investigated the antimicrobial, biofilm inhibitory, and antioxidant activities of bioactive compounds produced by the bacterial endophyte Serratia marcescens AI-N-1, isolated from A. indica. Crude extracts of S. marcescens showed strong antimicrobial activity against Bacillus subtilis (79.79% inhibition) and Salmonella typhi (77.04% inhibition) at 5 mg/ml. In addition, most extracts also displayed potent biofilm inhibition (>80%) against both pathogens, comparable to the positive control baicalein (P < 0.05). Antioxidant assays revealed high radical scavenging activity, with the supernatant extract obtained after 2 days of culture exhibiting the strongest effect (DPPH: 86.61% at 0.1 mg/ml; ABTS: 99.64% at 0.1 mg/ml). Online HPLC-ABTS(+) analysis identified serranticin as a major contributor to these antioxidant effects. HR-MS/MS profiling further revealed prodigiosin, serratamolides, and serranticin, along with putative novel lipopeptides and other metabolites, as key bioactive compounds. To our knowledge, this is the first report of a Serratia endophyte from A. indica in southwest Nigeria with combined antimicrobial, antibiofilm, and antioxidant activities, as well as the discovery of putative new lipopeptides. These findings highlight endophytic bacteria from Nigerian medicinal plants as promising sources of novel antimicrobial and antioxidant agents for pharmaceutical development. English 한국미생물·생명공학회 Chemical Profiling and Antibacterial, Anti-Biofilm, and Antioxidant Activities of Endophytic Serratia marcescens AI-N-1 from Azadirachta indica Article 10.4014/jmb.2508.08044 1 Journal of Microbiology and Biotechnology, v.35, pp.1 - 13 Journal of Microbiology and Biotechnology 35 1 13 Y scie scopus kci ART003270696 001629183700010 2-s2.0-105023334509 Biotechnology & Applied Microbiology Microbiology Biotechnology & Applied Microbiology Microbiology Article PRODIGIOSIN SOLVENT Bacterial endophyte Serratia marcescens bioactive compounds MS/MS based chemical profiling