Park, Hana Yang, Soyoun Lee, Dongwoo Son, Junghyun 2025-12-30T01:30:22Z 2025-12-30T01:30:22Z 2025-11-24 2025-11-20 https://pubs.kist.re.kr/handle/201004/153908 Background: Fecal microbiota transplantation (FMT) has emerged as a therapeutic strategy to restore gut microbial balance in gastrointestinal disorders. While alterations in gut microbiota and metabolites are known to influence disease progression and treatment outcomes, longitudinal metabolomics data following FMT remain limited. Methods: Untargeted metabolomics was performed using a Thermo Scientific Q Exactive LC–MS/MS system. Fecal samples from three time points—healthy donor, pre-FMT, and post-FMT—were collected and analyzed. Fecal samples (0.1 g wet; 20–40 mg freeze-dried) were processed using an ACN/MeOH (8:2, v/v) extraction at −20 °C, followed by concentration (≤30 °C) and reconstitution for LC–MS/MS analysis in positive and negative ionization modes. Heptadecanoic acid served as the internal standard. Results: Distinct metabolomic profiles were observed across clinical stages. Multivariate analysis revealed clear separation between pre- and post-FMT samples. Differential metabolite analysis indicated changes in multiple pathways, particularly amino acid and lipid metabolism, suggesting treatment-related metabolic shifts alongside disease-associated alterations. Conclusion: This study demonstrates the feasibility of longitudinal metabolomics profiling in FMT and reveals systemic metabolic changes across treatment stages. These findings provide mechanistic insights into host–microbiome–metabolite interactions and highlight the potential of metabolomics for identifying biomarkers of therapeutic response in FMT. English The Korean Society of Analytical Sciences Untargeted LC–MS/MS-Based Metabolomics Across Clinical Stages of Fecal Microbiota Transplantation Conference 2 제 75회 한국분석과학회 추계 학술대회 (The 75th Biannual Conference for The Korean Society of Analytical Sciences) 제 75회 한국분석과학회 추계 학술대회 (The 75th Biannual Conference for The Korean Society of Analytical Sciences) KO The K Hotel (경주) 2025-11-19 제75 회 한국분석과학회 추계 학술대회 초록집