Kounghwa Youn Soyeon Yoo Il Kwon Hwang Hyunchae Gil Hayoung Park Haein Lee Sunmin Oh Gyochang Keum Eun-Kyoung Bang 2026-01-02T07:30:23Z 2026-01-02T07:30:23Z 2025-12-31 2025-12 https://pubs.kist.re.kr/handle/201004/153950 Lipid nanoparticle (LNP) platforms are promising for mRNA therapeutics; however, while highly effective in vivo, commercial mRNA-LNPs show reduced transfection efficiency in vitro under serum-starved conditions, limiting mechanistic studies and cell engineering applications. Here, we present a new standard for in vitro transfection of mRNA-LNPs, including cell culture preparation, mRNA-LNP treatment in complete media, and mRNA expression level quantification. This protocol improves efficiency and reproducibility in in vitro screening and ensures consistency in the assessment of mRNA-LNPs. English Cell Press Protocol for in vitro transfection of mRNA-encapsulated lipid nanoparticles Article 10.1016/j.xpro.2025.104159 1 STAR Protocols, v.6, no.4 STAR Protocols 6 4 Y scopus 2-s2.0-105020957837