Lee, Jiyoung Yoon, Jaeeun Park, Ki Hong Lee, Juyun Hwang, Jin Hyun Na, Jongbeom Kim, Hojun Kim, Mijin Kim, Seon Joon Yun, Hongseok Oh, Taegon 2026-01-13T07:30:17Z 2026-01-13T07:30:17Z 2026-01-12 2025-12 1530-6984 https://pubs.kist.re.kr/handle/201004/153989 DNA immobilization on nanoparticle surfaces enables programmable assembly, gene sensing, and intracellular delivery. However, dense, direct functionalization of atomically thin two-dimensional (2D) materials remains challenging due to their inert basal planes. In contrast, 2D transition metal carbides (MXenes) possess highly polar, chemically active surfaces terminated with −OH, −O–, and −F groups, offering a unique platform for biofunctionalization. Herein, we exploit MXene’s surface chemistry for robust DNA grafting via designing a bifunctional catechol- and azide-terminated ligand. The catechol moiety anchors strongly to the MXene surface, while the azide group enables strain-promoted cycloaddition with dibenzocyclooctyne-terminated DNA. The resulting 2D DNA brush exhibits a high grafting density, evidenced by sequence-controlled self-assembly of MXene flakes and heteroassembly with complementary Au nanoparticles. This work presents a simple, effective strategy for producing colloidal two-dimensional nucleic acid brushes, establishing a versatile platform for further exploration of such bioactive nanobrush structures in the fields of nanoscience and biotechnology. English American Chemical Society Two-Dimensional Nucleic Acid Brushes on Colloidal MXene Sheets Article 10.1021/acs.nanolett.5c04642 1 Nano Letters, v.25, no.52, pp.18016 - 18025 Nano Letters 25 52 18016 18025 N scie scopus 001638350800001 2-s2.0-105026201065 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article SURFACE FUNCTIONALIZATION DNA NANOPARTICLES NANOSHEETS Surface chemistry DNA conjugation Two-dimensional material Self-assembly MXene Surface functionalization