Shin, Seung-cheol Hahm, Yale Jeong, Yeju Kim, Yup Park, Junsik Yang, Ji Hun Kim, Jin-A Won, Jihee Chung, Seok Lee, Jung-Yun 2026-02-04T05:30:41Z 2026-02-04T05:30:41Z 2026-02-02 2026-02 1473-0197 https://pubs.kist.re.kr/handle/201004/154188 The endometrium is the uterine lining that supports implantation and pregnancy. Existing in vitro systems only partly capture epithelial structure and function. We built a microfluidic model of the human endometrial epithelium using patient-derived organoids and defined a parameterized device and ECM conditions that yield a stable, polarized monolayer on chip. We specify the geometry, surface treatments, and collagen-based hydrogel or coating conditions, and we link these parameters to epithelial morphology and barrier integrity readouts. The epithelial layer maintains histologic features and endometrium-relevant markers and shows hormone-responsive transcript profiles. We quantify donor-to-donor variability across two donors and use it as a design constraint for reproducible culture. Because stromal and immune components shape the reproductive microenvironment, we will extend this platform to modular multicellular co-cultures that incorporate these elements. English Royal Society of Chemistry Formation of an endometrial epithelial monolayer in a microfluidic device with human tissue-derived endometrial organoids Article 10.1039/d5lc00278h 1 Lab on a Chip, v.26, no.4, pp.830 - 841 Lab on a Chip 26 4 830 841 N scie scopus 2-s2.0-105026574305 Biochemical Research Methods Chemistry, Multidisciplinary Chemistry, Analytical Nanoscience & Nanotechnology Instruments & Instrumentation Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Instruments & Instrumentation Article; Early Access PROGESTERONE-RECEPTOR EXPRESSION MECHANICAL-PROPERTIES COLLAGEN GELS LONG-TERM ESTROGEN ALPHA