Kim, Tae-Hun Park, Jae-Yeon Kwon, Soonil Jung, Jaeyong Sung, Jeong Soo Han, Dong-Ho Kang, Min-Jung Jose, Joachim Shin, Hyun-Jin Pyun, Jae-Chul 2026-02-19T04:30:51Z 2026-02-19T04:30:51Z 2026-02-19 2026-04 1618-2642 https://pubs.kist.re.kr/handle/201004/154279 Mimotopes of the porcine epidemic diarrhea virus (PEDV) spike protein (SP) were screened from a Fv-antibody library, and the neutralizing activity of screened mimotopes was analyzed using plaque assay and docking simulation. The screened Fv-antibody (mimotopes) corresponded to the variable region of the heavy chain of immunoglobulin G, composed of three complementarity-determining regions (CDRs) and four framework regions. The Fv-antibody library was constructed by randomizing the amino acid sequence of CDR3 and expressed on the outer membrane of E. coli using auto-display technology. Monoclonal anti-PEDV SP antibody was used as probes to screen Fv-antibodies mimicking PEDV SP from the library. Two screened Fv-antibodies (mimotopes of PEDV SP) with binding affinity to the monoclonal antibody were expressed as soluble proteins, and their binding affinity was estimated using a surface plasmon resonance biosensor. The neutralizing activity of PEDV SP mimotopes to prevent PEDV infection was calculated using a plaque assay based on the cytopathic effect. Additionally, molecular docking simulations were performed to examine the interactions of PEDV SP mimotopes with ACE2 receptor as well as APN which had been considered infection-related receptors for coronavirus. English Springer Verlag Neutralizing activity analysis of mimotopes against porcine epidemic diarrhea virus (PEDV) spike protein Article 10.1007/s00216-026-06339-y 1 Analytical and Bioanalytical Chemistry, v.418, pp.2135 - 2145 Analytical and Bioanalytical Chemistry 418 2135 2145 N scie scopus 2-s2.0-105028640137 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article; Early Access SITE-DIRECTED MUTAGENESIS INFECTION DIAGNOSIS EFFICACY VACCINE BINDING DOCKING Porcine epidemic diarrhea virus (PEDV) Fv-antibody library Mimotope Neutralizing activity