Cho, Yeongseon Kim, Hong Nam 2026-03-03T09:00:14Z 2026-03-03T09:00:14Z 2026-02-03 2025-05 0271-678X https://pubs.kist.re.kr/handle/201004/154391 Microplastics, especially those in the micrometer range, can enter the human body via ingestion, inhalation, and skin contact. Recent studies suggest that they may affect the central nervous system (CNS) by crossing the blood-brain barrier (BBB), though the mechanisms behind their uptake and toxicity remain unclear. In this study, we used an engineered 3D human BBB model to evaluate the size-dependent uptake and cytotoxicity of polystyrene microparticles. Our results showed that 0.2 μm particles had significantly higher cellular uptake and transendothelial transport than 1.0 μm particles, leading to greater BBB permeability and cellular damage. After 24 hours of exposure, permeability increased 15.6-fold for 0.2 μm and 2-fold for 1.0 μm particles. At 72 hours, these values increased to 27.3-fold and 4.5-fold, respectively, compared to control. Furthermore, microplastic exposure after TNF-α stimulation led to even greater BBB absorption and damage, suggesting inflammation exacerbates toxicity. Importantly, the toxic effects differed between conventional 2D cultures and 3D BBB models, underscoring the relevance of physiologically representative systems in environmental toxicity assessments. Our findings highlight the potential for microplastics to compromise the BBB and suggest an urgent need for further investigation into their neurological impacts. English 대한기계학회 Evaluation of Size-dependent Uptake, Transport and Cytotoxicity of Polystyrene Microplastic in a Blood-Brain Barrier (BBB) Model 혈액-뇌 장벽(BBB) 모델에서 폴리스티렌 미세 플라스틱의 크기 의존적 흡수, 운반 및 세포독성 평가 Conference 10.1186/s40580-024-00448-z 2 대한기계학회 바이오공학부문 2025년 춘계학술대회, v.45, no.1, pp.178 대한기계학회 바이오공학부문 2025년 춘계학술대회 45 1 178 178 KO 부산 벡스코 2025-05-21 대한기계학회 바이오공학부문 2025년도 춘계학술대회 초록집 001610844402092