Kim, Seong Yeol Thangam, Ramar Kang, Nayeon Hong, Hyunsik Kim, Chowon Lee, Sungkyu Son, Subin Lee,HyunJeong Kyong-Ryol Tag Sunhong, Min Jeong, Daun Hwang, Jangsun KIM KANGHYEON Kim, Dahee Kim, Yuri Joo, Jinmyoung Kim, Bong Hoon Zhu, Yangzhi Park, Sung­Gyu Song, Hyun-Cheol Sun, Wujin Ahn, Jae Pyoung Jang, Woo Young Paulmurugan, Ramasamy Kim, Hong­Kyu Kim, Jong Seung Kang, Heemin 2024-01-12T02:30:55Z 2024-01-12T02:30:55Z 2023-02-15 2023-04 1616-301X https://pubs.kist.re.kr/handle/201004/75754 Extracellular matrix (ECM) proteins containing cell-attachable Arg-Gly-Asp (RGD) sequences exhibit variable bridging and non-bridging in fibronectin-collagen and laminin-collagen complexes that can regulate inflammation, tissue repair, and wound healing. In this study, linking molecule-mediated conjugation of 1D magnetic nanocylinders (MNCs) to material surfaces pre-decorated with gold nanospheres (GNSs) is performed, thereby yielding RGD-coated MNCs (RGD-MNCs) over RGD-coated GNSs (RGD-GNSs) in a non-bridging state. The RGD-MNCs are drawn closer to the RGD-GNSs via magnetic field-mediated compression of the linking molecules to establish the bridging between them. Relative proportion of the RGD-MNCs to the RGD-GNSs is optimized to yield effective remote stimulation of integrin binding to variably bridged RGDs similar to that of invariably bridged RGDs used as a control group. Remote manipulation of the RGD bridging facilitates the attachment structure assembly of macrophages that leads to pro-healing/anti-inflammatory phenotype acquisition. In contrast, the non-bridged RGDs inhibited macrophage attachment that acquired pro-inflammatory phenotypes. The use of various nanomaterials in constructing heterogeneous RGD-coated materials can further offer various modes in remote switching of RGD bridging and non-bridging to understand dynamic integrin-mediated modulation of macrophages that regulate immunomodulatory responses, such as foreign body responses, tissue repair, and wound healing. English John Wiley & Sons Ltd. Modulation of Macrophages by In Situ Ligand Bridging Article 10.1002/adfm.202215166 1 Advanced Functional Materials, v.33, no.16 Advanced Functional Materials 33 16 N scie scopus 000929290700001 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article T-CELL-ACTIVATION DUAL ROLES ADHESION FIBRONECTIN POLARIZATION COLLAGEN BINDING INFLAMMATION RGD NEUTROPHILS in situ bridging ligand bridging macrophage adhesions macrophage polarization remote controls