Seo Su Yeong Jang, Minjeong Kim, Hwieun Sung, Jong Hwan Choi, Nakwon Lee, Kangwon Kim, Hong Nam 2024-01-12T02:31:44Z 2024-01-12T02:31:44Z 2023-01-11 2023-03 1616-301X https://pubs.kist.re.kr/handle/201004/75781 Air pollution induces neurodegeneration, including cognitive deficits, neuroinflammation, and disruption of the blood-brain barrier. The mechanisms underlying air pollution-mediated neurodegeneration have not yet been fully elucidated given the limited knowledge on intercellular interactions. A brain-on-a-chip platform is presented comprising neurons, glia, and brain endothelial cells (bECs; neuro-glia-vascular, NGV) and diesel exhaust particle (DEP)-induced neurodegeneration is evaluated with a particular focus on the intercellular interactions. DEP exposure in the NGV model yields Alzheimer's disease-like signatures, including amyloid beta accumulation, tau phosphorylation, hydrogen peroxide (H2O2)/reactive oxygen species (ROS) production, and neuronal cell death. bEC-secreted granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates microglial activation and the overproduction of H2O2/ROS in microglia, suggesting that the bEC-microglia-neuron is a neurodegeneration cascade. Pharmacological inhibition at each step of the cascade, including GM-CSF neutralization, microglial activation suppression, and ROS scavenging, prohibits neurodegeneration in the NGV model. Therefore, intercellular interactions should be further studied of air pollution-induced neurodegeneration. English John Wiley & Sons Ltd. Neuro-Glia-Vascular-on-a-Chip System to Assess Aggravated Neurodegeneration via Brain Endothelial Cells upon Exposure to Diesel Exhaust Particles Article 10.1002/adfm.202210123 1 Advanced Functional Materials, v.33, no.12 Advanced Functional Materials 33 12 N scie scopus 000905312800001 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article AIR-POLLUTION PARTICULATE MATTER MICROGLIA LIMITATIONS TIME blood-brain barriers brain endothelial cells diesel exhaust particles granulocyte-macrophage colony-stimulating factor neurodegeneration