Han, Sukmin Seokyoung Bang Kim, Hong Nam Choi, Nakwon Kim, Sung Hyun 2024-01-12T02:33:19Z 2024-01-12T02:33:19Z 2023-02-18 2023-01 1756-6606 https://pubs.kist.re.kr/handle/201004/75849 The central nervous system is organized into different neural circuits, each with particular functions and properties. Studying neural circuits is essential to understanding brain function and neuronal diseases. Microfluidic systems are widely used for reconstructing and studying neural circuits but still need improvement to allow modulation and monitoring of the physiological properties of circuits. In this study, we constructed an improved microfluidic device that supports the electrical modulation of neural circuits and proper reassembly. We demonstrated that our microfluidic device provides a platform for electrically modulating and monitoring the physiological function of neural circuits with genetic indicators for synaptic functionality in corticostriatal (CStr) circuits. In particular, our microfluidic device measures activity-driven Ca2+ dynamics using Ca2+ indicators (synaptophysin-GCaMP6f and Fluo5F-AM), as well as activity-driven synaptic transmission and retrieval using vGlut-pHluorin. Overall, our findings indicate that the improved microfluidic platform described here is an invaluable tool for studying the physiological properties of specific neural circuits. English BioMed Central Modulating and monitoring the functionality of corticostriatal circuits using an electrostimulable microfluidic device Article 10.1186/s13041-023-01007-z 1 Molecular Brain, v.16, no.1 Molecular Brain 16 1 Y scie scopus 000918320800002 Neurosciences Neurosciences & Neurology Article Microfluidic device Corticostriatal (CStr) circuit SynapseCa2+ dynamics Action potential Synaptic transmission